دورية أكاديمية
Efficient synthesis 1,4-cyclohexanedicarboxaldehyde by an engineered alcohol oxidase
العنوان: | Efficient synthesis 1,4-cyclohexanedicarboxaldehyde by an engineered alcohol oxidase |
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المؤلفون: | Yaqi Cheng, Wei Song, Xiulai Chen, Cong Gao, Jia Liu, Liang Guo, Meng Zhu, Liming Liu, Jing Wu |
المصدر: | Bioresources and Bioprocessing, Vol 9, Iss 1, Pp 1-14 (2022) |
بيانات النشر: | SpringerOpen, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Technology LCC:Chemical technology LCC:Biotechnology |
مصطلحات موضوعية: | 1,4-Cyclohexanedicarboxaldehyde, Alcohol oxidase, Primary alcohol oxidation reaction, Protein engineering, Technology, Chemical technology, TP1-1185, Biotechnology, TP248.13-248.65 |
الوصف: | Abstract In this study, we selected and engineered a flavin adenine dinucleotide (FAD)-dependent alcohol oxidase (AOX) to produce 1,4-cyclohexanedicarboxaldehyde (CHDA), an initial raw material for spiral compounds, from 1,4-cyclohexanedimethanol (CHDM). First, the structure of alcohol oxidase from Arthrobacter cholorphenolicus (AcCO) was analyzed, and the mechanism of AcCO-catalyzed primary alcohol oxidation was elucidated, demonstrating that the energy barrier of the hydride (H−) transfer (13.4 kcal·mol−1 and 20.4 kcal·mol−1) decreases the catalytic efficiency of the primary alcohol oxidation reaction. Therefore, we designed a protein engineering strategy to adjust the catalytically active conformation to shorten the distance of hydride (H−) transfer and further decreased the core energy barrier. Following this strategy, variant W4 (S101A/H351V/N378S/Q329N) was obtained with 112.5-fold increased catalytic efficiency to produce CHDA compared to that of the wild-type strain. The 3 L scale preparation of CHDA reached a titer up to 29.6 g·L−1 with a 42.2% yield by an Escherichia coli whole-cell catalyst, which demonstrates the potential of this system for industrial application. Graphical Abstract |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2197-4365 |
العلاقة: | https://doaj.org/toc/2197-4365Test |
DOI: | 10.1186/s40643-022-00570-y |
الوصول الحر: | https://doaj.org/article/a7771bd53b0c48b891a4988a3714761dTest |
رقم الانضمام: | edsdoj.7771bd53b0c48b891a4988a3714761d |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 21974365 |
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DOI: | 10.1186/s40643-022-00570-y |