دورية
Mechanics of Individual Keratin Bundles in Living Cells
| العنوان: | Mechanics of Individual Keratin Bundles in Living Cells |
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| المؤلفون: | Nolting, Jens-Friedrich, Möbius, Wiebke, Köster, Sarah |
| المصدر: | Biophysical Journal; December 2014, Vol. 107 Issue: 11 p2693-2699, 7p |
| مستخلص: | Along with microtubules and microfilaments, intermediate filaments are a major component of the eukaryotic cytoskeleton and play a key role in cell mechanics. In cells, keratin intermediate filaments form networks of bundles that are sparser in structure and have lower connectivity than, for example, actin networks. Because of this, bending and buckling play an important role in these networks. Buckling events, which occur due to compressive intracellular forces and cross-talk between the keratin network and other cytoskeletal components, are measured here in situ. By applying a mechanical model for the bundled filaments, we can access the mechanical properties of both the keratin bundles themselves and the surrounding cytosol. Bundling is characterized by a coupling parameter that describes the strength of the linkage between the individual filaments within a bundle. Our findings suggest that coupling between the filaments is mostly complete, although it becomes weaker for thicker bundles, with some relative movement allowed. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00063495 15420086 |
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| DOI: | 10.1016/j.bpj.2014.10.039 |