Free-Radical-Mediated Formation of Trans-Cardiolipin Isomers, Analytical Approaches for Lipidomics and Consequences of the Structural Organization of Membranes
| العنوان: | Free-Radical-Mediated Formation of Trans-Cardiolipin Isomers, Analytical Approaches for Lipidomics and Consequences of the Structural Organization of Membranes |
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| المؤلفون: | Marinella Roberti, Chryssostomos Chatgilialoglu, Gessica Batani, Anna Sansone, Cesare Meliota, Carla Ferreri, Fabrizio Vetica |
| المساهمون: | Vetica F., Sansone A., Meliota C., Batani G., Roberti M., Chatgilialoglu C., Ferreri C. |
| المصدر: | Biomolecules Volume 10 Issue 8 Biomolecules, Vol 10, Iss 1189, p 1189 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | linoleic acid, Chromatography, Gas, Stereochemistry, Cardiolipins, lcsh:QR1-502, free radicals, 010402 general chemistry, 01 natural sciences, Biochemistry, lcsh:Microbiology, Article, Mitochondria, Heart, Fenton reaction, 03 medical and health sciences, chemistry.chemical_compound, cis-trans isomerization, Isomerism, Free radical, Lipidomics, Cardiolipin, Animals, Inner mitochondrial membrane, Molecular Biology, 030304 developmental biology, Mercaptoethanol, chemistry.chemical_classification, liposome dimension, 0303 health sciences, Photolysis, Fatty acid, γ-irradiation, Cis trans isomerization, 0104 chemical sciences, Kinetics, Membrane, chemistry, Mitochondrial Membranes, Cattle, Lipid Peroxidation, cardiolipin, Isomerization |
| الوصف: | Free-radical-mediated processes, such as peroxidation, isomerization and hydrogenation affecting fatty acid integrity and biological functions, have a trans-disciplinary relevance. Cardiolipins (CL, (1,3-diphosphatidyl-sn-glycerol)) and tetra-linoleoyl-CL are complex phospholipids, exclusively present in the Inner Mitochondrial Membrane (IMM) lipids, where they maintain membrane integrity and regulate enzyme functionalities. Peroxidation pathways and fatty acid remodeling are known causes of mitochondrial disfunctions and pathologies, including cancer. Free-radical-mediated isomerization with the change of the cis CL into geometrical trans isomers is an unknown process with possible consequences on the supramolecular membrane lipid organization. Here, the formation of mono-trans CL (MT-CL) and other trans CL isomers (T-CL) is reported using CL from bovine heart mitochondria and thiyl radicals generated by UV-photolysis from 2-mercaptoethanol. Analytical approaches for CL isomer separation and identification via 1H/13C NMR are provided, together with the chemical study of CL derivatization to fatty acid methyl esters (FAME), useful for lipidomics and metabolomics research. Kinetics information of the radical chain isomerization process was obtained using &gamma irradiation conditions. The CL isomerization affected the structural organization of membranes, as tested by the reduction in unilamellar liposome diameter, and accompanied the well-known process of oxidative consumption induced by Fenton reagents. These results highlight a potential new molecular modification pathway of mitochondrial lipids with wide applications to membrane functions and biological consequences. |
| وصف الملف: | application/pdf; STAMPA |
| اللغة: | English |
| تدمد: | 2218-273X |
| DOI: | 10.3390/biom10081189 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b0f70d47f3c726f7f4974e29dbddbf8Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7b0f70d47f3c726f7f4974e29dbddbf8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2218273X |
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| DOI: | 10.3390/biom10081189 |