Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper
| العنوان: | Novel Bradykinin-Potentiating Peptides and Three-Finger Toxins from Viper Venom: Combined NGS Venom Gland Transcriptomics and Quantitative Venom Proteomics of the Azemiops feae Viper |
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| المؤلفون: | Rustam H. Ziganshin, Igor A. Dyachenko, Vladislav V. Babenko, Anh Ngoc Hoang, Yuri N. Utkin, Victor I. Tsetlin, Maxim N. Zhmak, Elvira R. Shaykhutdinova, Vladislav G. Starkov, Arkady N. Murashev, Christoph Weise |
| المصدر: | Biomedicines, Vol 8, Iss 249, p 249 (2020) Biomedicines Volume 8 Issue 8 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, VIPeR, Medicine (miscellaneous), venom, Venom, Phospholipase, Proteomics, Viper Venoms, complex mixtures, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, proteomics, Viperidae, bradykinin-potentiating peptides, biology.animal, lcsh:QH301-705.5, venom gland, 030102 biochemistry & molecular biology, biology, 500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie), transcriptomic, biology.organism_classification, 030104 developmental biology, Biochemistry, lcsh:Biology (General), Feae’s viper, Proteome, Azemiops feae, Azemiopinae, 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften, three-finger toxins |
| الوصف: | Feae&rsquo s viper Azemipos feae belongs to the Azemiopinae subfamily of the Viperidae family. The effects of Viperidae venoms are mostly coagulopathic with limited neurotoxicity manifested by phospholipases A2. From A. feae venom, we have earlier isolated azemiopsin, a novel neurotoxin inhibiting the nicotinic acetylcholine receptor. To characterize other A. feae toxins, we applied label-free quantitative proteomics, which revealed 120 unique proteins, the most abundant being serine proteinases and phospholipases A2. In total, toxins representing 14 families were identified, among which bradykinin-potentiating peptides with unique amino acid sequences possessed biological activity in vivo. The proteomic analysis revealed also basal (commonly known as non-conventional) three-finger toxins belonging to the group of those possessing neurotoxic activity. This is the first indication of the presence of three-finger neurotoxins in viper venom. In parallel, the transcriptomic analysis of venom gland performed by Illumina next-generation sequencing further revealed 206 putative venom transcripts. Together, the study unveiled the venom proteome and venom gland transciptome of A. feae, which in general resemble those of other snakes from the Viperidae family. However, new toxins not found earlier in viper venom and including three-finger toxins and unusual bradykinin-potentiating peptides were discovered. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2227-9059 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4438a94e306996b26b01f85e80275b64Test https://www.mdpi.com/2227-9059/8/8/249Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4438a94e306996b26b01f85e80275b64 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22279059 |
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