التفاصيل البيبلوغرافية
| العنوان: |
Three-dimensional virtual histology of the cerebral cortex based on phase-contrast X-ray tomography |
| المؤلفون: |
Eckermann, Marina, van der Meer, Franziska, Cloetens, Peter, Ruhwedel, Torben, Möbius, Wiebke, Stadelmann, Christine, Salditt, Tim |
| المصدر: |
Biomedical Optics Express; December 2021, Vol. 12 Issue: 12 p7582-7598, 17p |
| مستخلص: |
In this work, we optimize the setups and experimental parameters of X-ray phase-contrast computed-tomography for the three-dimensional imaging of the cyto- and myeloarchitecture of cerebral cortex, including both human and murine tissue. We present examples for different optical configurations using state-of-the art synchrotron instruments for holographic tomography, as well as compact laboratory setups for phase-contrast tomography in the direct contrast (edge-enhancement) regime. Apart from unstained and paraffin-embedded tissue, we tested hydrated tissue, as well as heavy metal stained and resin-embedded tissue using two different protocols. Further, we show that the image quality achieved allows to assess the neuropathology of multiple sclerosis in a biopsy sample collected during surgery. |
| قاعدة البيانات: |
Supplemental Index |
Full Text Finder