Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation
| العنوان: | Amelioration of LPS-Induced Inflammation Response in Microglia by AMPK Activation |
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| المؤلفون: | Chin Chen Chen, Shao-Hsuan Kao, Yao Jen Liang, Cheng-Yi Kuo, Chun Fang Huang, Ching Yi Cheng, Jiun Tsai Lin, Yi Fang Cheng, Han Min Chen |
| المصدر: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| بيانات النشر: | Hindawi Publishing Corporation, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Lipopolysaccharides, Transcriptional Activation, medicine.medical_specialty, Article Subject, lcsh:Medicine, Inflammation, AMP-Activated Protein Kinases, Nitric Oxide, General Biochemistry, Genetics and Molecular Biology, Dinoprostone, Nitric oxide, Cell Line, chemistry.chemical_compound, AMP-activated protein kinase, Internal medicine, medicine, Humans, Protein kinase A, General Immunology and Microbiology, biology, Microglia, Plant Extracts, lcsh:R, AMPK, General Medicine, Cell biology, Nitric oxide synthase, medicine.anatomical_structure, Endocrinology, chemistry, biology.protein, Phosphorylation, medicine.symptom, Energy Metabolism, Sasa, Research Article |
| الوصف: | Adenosine 5′-monophosphate-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis via modulating metabolism of glucose, lipid, and protein. In addition to energy modulation, AMPK has been demonstrated to associate with several important cellular events including inflammation. The results showed that ENERGI-F704 identified from bamboo shoot extract was nontoxic in concentrations up to 80 μM and dose-dependently induced phosphorylation of AMPK (Thr-172) in microglia BV2 cells. Our findings also showed that the treatment of BV2 with ENERGI-F704 ameliorated the LPS-induced elevation of IL-6 and TNF-αproduction. In addition, ENERGI-F704 reduced increased production of nitric oxide (NO) and prostaglandin E2 (PGE2) via downregulating the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2), respectively. Moreover, ENERGI-F704 decreased activated nuclear translocation and protein level of NF-κB. Inhibition of AMPK with compound C restored decreased NF-κB translocation by ENERGI-F704. In conclusion, ENERGI-F704 exerts inhibitory activity on LPS-induced inflammation through manipulating AMPK signaling and exhibits a potential therapeutic agent for neuroinflammatory disease. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4821f73aadbb816556a73490fb0ec3e9Test http://europepmc.org/articles/PMC4084681Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4821f73aadbb816556a73490fb0ec3e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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