دورية
PNJ scaffolds promote microenvironmental regulation of glioblastoma stem-like cell enrichment and radioresistanceElectronic supplementary information (ESI) available. See DOI: 10.1039/d0bm01169j
العنوان: | PNJ scaffolds promote microenvironmental regulation of glioblastoma stem-like cell enrichment and radioresistanceElectronic supplementary information (ESI) available. See DOI: 10.1039/d0bm01169j |
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المؤلفون: | Heffernan, John M., McNamara, James B., Vernon, Brent L., Mehta, Shwetal, Sirianni, Rachael W. |
المصدر: | Biomaterials Science; 2022, Vol. 10 Issue: 3 p819-833, 15p |
مستخلص: | Glioblastoma (GBM) brain tumors contain a subpopulation of self-renewing multipotent Glioblastoma stem-like cells (GSCs) that are believed to drive the near inevitable recurrence of GBM. We previously engineered temperature responsive scaffolds based on the polymer poly(N-isopropylacrylamide-co-Jeffamine M-1000 acrylamide) (PNJ) for the purpose of enriching GSCs in vitrofrom patient-derived samples. Here, we used PNJ scaffolds to study microenvironmental regulation of self-renewal and radiation response in patient-derived GSCs representing classical and proneural subtypes. GSC self-renewal was regulated by the composition of PNJ scaffolds and varied with cell type. PNJ scaffolds protected against radiation-induced cell death, particularly in conditions that also promoted GSC self-renewal. Additionally, cells cultured in PNJ scaffolds exhibited increased expression of the transcription factor HIF2α, which was not observed in neurosphere culture, providing a potential mechanistic basis for differences in radio-resistance. Differences in PNJ regulation of HIF2α in irradiated and untreated conditions also offered evidence of stem plasticity. These data show PNJ scaffolds provide a unique biomaterial for evaluating dynamic microenvironmental regulation of GSC self-renewal, radioresistance, and stem plasticity. |
قاعدة البيانات: | Supplemental Index |
تدمد: | 20474830 20474849 |
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DOI: | 10.1039/d0bm01169j |