| المساهمون: |
Evangelopoulos, Michael, Parodi, Alessandro, Martinez, Jonathan O., Yazdi, Iman K., Cevenini, Armando, van de Ven, Anne L., Quattrocchi, Nicoletta, Boada, Christian, Taghipour, Nima, Corbo, Claudia, Brown, Brandon S., Scaria, Shilpa, Liu, Xuewu, Ferrari, Mauro, Tasciotti, Ennio, Evangelopoulos, M, Parodi, A, Martinez, J, Yazdi, I, Cevenini, A, van de Ven, A, Quattrocchi, N, Boada, C, Taghipour, N, Corbo, C, Brown, B, Scaria, S, Liu, X, Ferrari, M, Tasciotti, E |
| مصطلحات موضوعية: |
opsonization, leukocyte membrane, Cell, Biocompatible Materials, animal cell, 02 engineering and technology, reactive oxygen metabolite, Mice, Nanoparticle, aspartate aminotransferase, Biomimetic Materials, drug delivery system, Macrophage, cell interaction, Leukolike Vector, comparative study, Cells, Cultured, tumor necrosis factor alpha, Systemic administration, creatinine, biomimetic material, Cell biology, Antibody opsonization, cytokine release, priority journal, nanocarrier, 0210 nano-technology, surface charge, in vitro study, Biocompatibility, Biophysics, Ceramics and Composite, Bioengineering, macrophage, Article, animal tissue, in vivo study, Biomaterials, zeta potential, 03 medical and health sciences, Nanovector, human, protein expression, mouse, human cell, Drug products, Biological materials, mononuclear phagocyte, Immunity, Innate, Cell membranes, 030104 developmental biology, Immunology, interleukin 1alpha, Nanoparticles, Comprehensive evaluation, 0301 basic medicine, interleukin 1beta, immunology, Bagg albino mouse, Biomimetics, Leukocytes, nanocapsule, animal, Multistage nanovector, innate immunity, Immunological effects, Mice, Inbred BALB C, Time-lapse microscopy, phagocytosis, 021001 nanoscience & nanotechnology, unclassified drug, medicine.anatomical_structure, liver function, Mechanics of Materials, Drug delivery, gamma interferon, medicine.symptom, leukocyte, time lapse imaging, alanine aminotransferase, Antigen-antibody reactions, animal experiment, transcription factor RelA, interleukin 6, Inflammation, Biology, interleukin 2, Nanocapsules, interleukin 3, evaluation study, medicine, Biomimicry, Animals, controlled study, Mechanics of Material, Immunobiology, cell culture, nonhuman, static electricity, Biomaterial, Biomimetic nanoparticles, internalization, 7 INGENIERÍA Y TECNOLOGÍA, drug effects, Ceramics and Composites, interleukin 12, interleukin 10 |
| الوصف: |
Recently, engineering the surface of nanotherapeutics with biologics to provide them with superior biocompatibility and targeting towards pathological tissues has gained significant popularity. Although the functionalization of drug delivery vectors with cellular materials has been shown to provide synthetic particles with unique biological properties, these approaches may have undesirable immunological repercussions upon systemic administration. Herein, we comparatively analyzed unmodified multistage nanovectors and particles functionalized with murine and human leukocyte cellular membrane, dubbed Leukolike Vectors (LLV), and the immunological effects that may arise in vitro and in vivo. Previously, LLV demonstrated an avoidance of opsonization and phagocytosis, in addition to superior targeting of inflammation and prolonged circulation. In this work, we performed a comprehensive evaluation of the importance of the source of cellular membrane in increasing their systemic tolerance and minimizing an inflammatory response. Time-lapse microscopy revealed LLV developed using a cellular coating derived from a murine (i.e., syngeneic) source resulted in an active avoidance of uptake by macrophage cells. Additionally, LLV composed of a murine membrane were found to have decreased uptake in the liver with no significant effect on hepatic function. As biomimicry continues to develop, this work demonstrates the necessity to consider the source of biological material in the development of future drug delivery carriers. © 2015. |
