In Vitro Evaluation of Textile Chitosan Scaffolds for Tissue Engineering using Human Bone Marrow Stromal Cells
| العنوان: | In Vitro Evaluation of Textile Chitosan Scaffolds for Tissue Engineering using Human Bone Marrow Stromal Cells |
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| المؤلفون: | Hartmut Worch, Sascha Heinemann, Anne Bernhardt, Thomas Hanke, Anja Lode, Christiane Heinemann |
| المصدر: | Biomacromolecules. 10:1305-1310 |
| بيانات النشر: | American Chemical Society (ACS), 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Stromal cell, Polymers and Plastics, Biocompatibility, Surface Properties, Cell Culture Techniques, Biocompatible Materials, Bone Marrow Cells, Cell Count, Bioengineering, Biomaterials, Chitosan, chemistry.chemical_compound, Tissue engineering, Materials Testing, Materials Chemistry, Humans, Particle Size, Cells, Cultured, Cell Proliferation, L-Lactate Dehydrogenase, Tissue Engineering, Tissue Scaffolds, biology, Textiles, Biomaterial, Cell Differentiation, Anatomy, Alkaline Phosphatase, chemistry, Cell culture, Osteocalcin, biology.protein, Alkaline phosphatase, Stromal Cells, Biomedical engineering |
| الوصف: | Textile chitosan fiber scaffolds were developed and tested in terms of biocompatibility for human bone marrow stromal cells (hBMSCs). A part of the scaffolds was further modified by coating with fibrillar collagen type I in order to biologize the surface. hBMSCs of two donors were used for cell culture experiments in vitro. Confocal laser scanning microscopy (CLSM) as well as scanning electron microscopy (SEM) revealed fast attachment and morphological adaptation of the cells on both the raw chitosan fibers and the collagen-coated scaffolds. Cells were osteogenically induced after 3 days and cultivated for up to 28 days on the scaffolds. Activity of lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) was analyzed to evaluate proliferation as well as osteogenic differentiation. We found a 3.5-6-fold increase in the cell number, whereas the collagen coating did not noticeably influence these factors. Osteogenic differentiation was confirmed by the course of ALP activity and immunostaining of osteocalcin. The feature of the collagen-coated as well as the raw chitosan fiber scaffolds to support attachment, proliferation, and differentiation of hBMSCs suggests a potential application of chitosan fibers and textile chitosan scaffolds for the tissue engineering of bone. |
| تدمد: | 1526-4602 1525-7797 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fad242de54b37f05fcd70e05960c822bTest https://doi.org/10.1021/bm900172mTest |
| رقم الانضمام: | edsair.doi.dedup.....fad242de54b37f05fcd70e05960c822b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15264602 15257797 |
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