Increased miRNA-518b inhibits trophoblast migration and angiogenesis by targeting EGR1 in early embryonic arrest†
| العنوان: | Increased miRNA-518b inhibits trophoblast migration and angiogenesis by targeting EGR1 in early embryonic arrest† |
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| المؤلفون: | Zhifu Zhi, Deng Lingjie, Lihong Pang, Wenmei Yang, Zhaoyi Lu, Xiaoli Jiang, Liling Liu |
| المصدر: | Biology of Reproduction. 101:664-674 |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, 0301 basic medicine, endocrine system, Angiogenesis, Cellular differentiation, EGR1, Neovascularization, Physiologic, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Pre-Eclampsia, Cell Movement, Pregnancy, microRNA, medicine, Humans, Gene silencing, Embryo Implantation, Early Growth Response Protein 1, 030219 obstetrics & reproductive medicine, Gene Expression Regulation, Developmental, Trophoblast, Cell Biology, General Medicine, Embryonic stem cell, Trophoblasts, Up-Regulation, Cell biology, Vascular endothelial growth factor, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Reproductive Medicine, chemistry, Case-Control Studies, Female |
| الوصف: | Evidence indicates that microRNAs (miRNAs) play essential roles in early embryonic development. The miRNA-518 family is a special biomarker of the placenta, and miRNA-518b is abnormally expressed in placental tissue in preeclampsia. Early growth response protein 1 (EGR1), a zinc finger transcriptional factor, plays an essential role in regulating cell differentiation, angiogenesis, and migration. Moreover, earlier studies have shown that EGR1 protein plays a key role in implantation. However, little is known about the role of miR-518b and EGR1 on early embryonic arrest (EEA) in humans. In our study, increased miR-518b along with decreased EGR1 was found in human villus tissues with EEA. Furthermore, we demonstrated by luciferase assay that miR-518b is a direct regulator of EGR1. After comparing the effect of silencing EGR1, vascular endothelial growth factor (VEGF) individually, and EGR1/VEGF in combination, we found that EGR1 can inhibit migration and angiogenesis of HTR-8 SVneo cells by decreasing the VEGF expression. Hypoxia plays an initial role in early embryonic development, and we found that hypoxia reduces the expression of miR-518b and increases the expression of EGR1 and VEGF to facilitate migration and angiogenesis in a hypoxic model of HTR-8/SVneo cell line. Our findings provide new insights into the role of miR-518b in EEA and implicate the potential application of miR-518b in the diagnosis and development of intervention for EEA. |
| تدمد: | 1529-7268 0006-3363 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::96c93056cdd48c8248cc54e74574e2b1Test https://doi.org/10.1093/biolre/ioz109Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....96c93056cdd48c8248cc54e74574e2b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15297268 00063363 |
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