دورية أكاديمية
Sex Chromosomes and Sex-Specific Molecular Markers in Indo-Pacific Combtooth Blennies (Blenniidae, Istiblennius )
| العنوان: | Sex Chromosomes and Sex-Specific Molecular Markers in Indo-Pacific Combtooth Blennies (Blenniidae, Istiblennius ) |
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| المؤلفون: | Hundt, Peter J., Liddle, Emma B., Nielsen, Stuart V., Pinto, Brendan J., Gamble, Tony |
| المصدر: | Biological Sciences Faculty Research and Publications |
| بيانات النشر: | e-Publications@Marquette |
| سنة النشر: | 2019 |
| المجموعة: | Marquette University: e-Publications@Marquette |
| مصطلحات موضوعية: | blenny, fish, RADseq, Endocrine disrupting chemicals, Biomonitoring, Biology |
| الوصف: | Sex-specific genetic markers, markers found in one sex but not the other, can be used to recognize a species’ sex chromosome system in cases where traditional karyotyping fails. Species with male-specific markers have an XX/XY system, while species with female-specific markers have a ZZ/ZW system. Here, we used data from restriction site-associated DNA sequencing, or RADseq, from 2 species of combtooth blenny, Istiblennius lineatus and I. steindachneri, to identify an excess of female-specific genetic markers, which points to ZZ/ZW sex chromosomes. We used PCR to validate the sex-specificity of one of these female-specific restriction site-associated DNA markers in an additional Istiblennius species, I. edentulus. We observed no sex-specific PCR amplification in 2 other Istiblennius species and 2 Blenniella species. This Istiblennius ZZ/ZW system, when combined with cytogenetic data from the literature illustrating an XX/XY system in Parablennius, establishes a transition between sex chromosome systems within Blenniidae. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://epublications.marquette.edu/bio_fac/918Test; http://dx.doi.org/10.3354/meps13082Test |
| DOI: | 10.3354/meps13082 |
| الإتاحة: | https://doi.org/10.3354/meps13082Test https://epublications.marquette.edu/bio_fac/918Test |
| رقم الانضمام: | edsbas.117497CB |
| قاعدة البيانات: | BASE |
| DOI: | 10.3354/meps13082 |
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