دورية أكاديمية
Interaction of human heat shock protein 70 with tumor-associated peptides
| العنوان: | Interaction of human heat shock protein 70 with tumor-associated peptides |
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| المؤلفون: | Pandya, Maya J., Bendz, Henriette, Manzenrieder, Florian, Noessner, Elfriede, Kessler, Horst, Buchner, Johannes, Issels, Rolf D. |
| المصدر: | Biological Chemistry |
| بيانات النشر: | Ludwig-Maximilians-Universität München |
| سنة النشر: | 2009 |
| المجموعة: | Open Access LMU (Ludwig-Maximilians-University Munich) |
| مصطلحات موضوعية: | Medizin, ddc:540 |
| الوصف: | Molecular chaperones of the heat shock protein 70 (Hsp70) family play a crucial role in the presentation of exogenous antigenic peptides by antigen-presenting cells (APCs). In a combined biochemical and immunological approach, we characterize the biochemical interaction of tumor-associated peptides with human Hsp70 and show that the strength of this interaction determines the efficacy of immunological cross-presentation of the antigenic sequences by APCs. A fluorescein-labeled cytosolic mammalian Hsc70 binding peptide is shown to interact with human Hsp70 molecules with high affinity (K(d)=0.58 mu M at 25 degrees C). Competition experiments demonstrate weaker binding by Hsp70 of antigenic peptides derived from the tumor-associated proteins tyrosinase (K(d)=32 mu M) and melanoma antigen recognized by T cells (MART-1) (K(d)=2.4 mu M). Adding a peptide sequence (pep70) with high Hsp70 binding affinity (K(d)=0.04 mu M) to the tumor-associated peptides enables them to strongly interact with Hsp70. Presentation of tumor-associated peptides by B cells resulting in T cell activation in vitro is enhanced by Hsp70 when the tumor-associated peptides contain the Hsp70 binding sequence. This observation has relevance for vaccine design, as augmented transfer of tumor-associated antigens to APCs is closely linked to the vaccine's efficacy of T cell stimulation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | Pandya, Maya J.; Bendz, Henriette; Manzenrieder, Florian; Noessner, Elfriede; Kessler, Horst; Buchner, Johannes; Issels, Rolf D. (2009): Interaction of human heat shock protein 70 with tumor-associated peptides. In: Biological Chemistry, Vol. 390, Nr. 4: S. 304-312 [PDF, 155kB]; https://epub.ub.uni-muenchen.de/17749/1/bc.2009.038.pdfTest; http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-17749-3Test; https://epub.ub.uni-muenchen.de/17749Test/ |
| DOI: | 10.1515/BC.2009.038 |
| الإتاحة: | https://doi.org/10.1515/BC.2009.038Test https://epub.ub.uni-muenchen.de/17749/1/bc.2009.038.pdfTest https://epub.ub.uni-muenchen.de/17749Test/ http://nbn-resolving.de/urn:nbn:de:bvb:19-epub-17749-3Test |
| حقوق: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively |
| رقم الانضمام: | edsbas.92D0013C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1515/BC.2009.038 |
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