Inhibition of L-Type Amino Acid Transporter Modulates the Expression of Cell Cycle Regulatory Factors in KB Oral Cancer Cells
| العنوان: | Inhibition of L-Type Amino Acid Transporter Modulates the Expression of Cell Cycle Regulatory Factors in KB Oral Cancer Cells |
|---|---|
| المؤلفون: | Yoshikatsu Kanai, Joo-Cheol Park, Woo-Cheol Shin, Ju-Hyun Park, Hitoshi Endou, Chun Sung Kim, In-Sung Moon, Byung-Kwon Lee, Sook-Young Lee, Do Kyung Kim, Hong Sung Chun, Joong-Ki Kook, Heung-Joong Kim |
| المصدر: | Biological and Pharmaceutical Bulletin. 33:1117-1121 |
| بيانات النشر: | Pharmaceutical Society of Japan, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Pharmacology, Cell cycle checkpoint, Amino Acid Transport Systems, biology, Cell growth, Blotting, Western, Pharmaceutical Science, Cell Cycle Proteins, General Medicine, Cell cycle, Molecular biology, chemistry.chemical_compound, chemistry, Cell culture, Cyclin-dependent kinase, Cell Line, Tumor, Cancer cell, biology.protein, Humans, Immunoprecipitation, Mouth Neoplasms, Cyclin-dependent kinase 6, Growth inhibition |
| الوصف: | The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at G1 phase by inhibiting cyclin D3-CDK6 complex whereas increasing expression of a CDK inhibitor p27. |
| تدمد: | 1347-5215 0918-6158 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bc49ba1fe90fef73d57c38c06328250Test https://doi.org/10.1248/bpb.33.1117Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4bc49ba1fe90fef73d57c38c06328250 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13475215 09186158 |
|---|