التفاصيل البيبلوغرافية
| العنوان: |
АНТИВОСПАЛИТЕЛЬНЫЕ СВОЙСТВА МЕТФОРМИНА В УСЛОВИЯХ КУЛЬТИВАЦИИ ПЕРВИЧНЫХ АСТРОЦИТОВ КРЫСЫ В СРЕДЕ С ПОВЫШЕННОЙ КОНЦЕНТРАЦИЕЙ ГЛЮКОЗЫ |
| المؤلفون: |
Горбатенко В.О., Горяинов С.В., Бабенко В.А., Плотников Е.Ю., Сергеева М.Г., Чистяков Д.В. |
| المصدر: |
Biokhimiya |
| المجموعة: |
RUDN University Open Repository (RUOR) |
| مصطلحات موضوعية: |
astrocytes, hyperglycemia, cytokines, oxylipins, ros, polyunsaturated fatty acids, anandamide, metformin, астроциты, гипергликемия, цитокины, оксилипины, Stat3, Erk, афк, полиненасыщенные жирные кислоты, анандамид, метформин |
| الوصف: |
Investigation of the relationship between inflammation and energy metabolism is important for understanding the biology of chronic noncommunicable diseases. The use of metformin, a drug for the treatment of diabetes, is considered as a promising direction for the treatment of neurodegenerative diseases and other neuropathology’s with an inflammatory component. Astrocytes play an important role in the regulation of energy metabolism and neuroinflammation; therefore, we studied the effect of metformin on the cellular responses of primary rat astrocytes cultured in a medium with high glucose concentration (incubation for 48 h, 22.5 mM). Lipopolysaccharide (LPS) was used as an inflammatory stimulus. The effects of metformin were assessed by changes in the expression of proinflammatory cytokines and the synthesis of oxylipins, detected by ultra-high-performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS). Changes at the intracellular level were assessed by analyzing the phosphorylation of ERK kinase and the transcription factor STAT3, cyclooxygenase 1 and 2 (COX) oxylipin synthesis enzymes. It was found that, regardless of glucose concentration, metformin reduces the LPS-stimulated release of cytokines IL-1β and IL-6, the activity of the transcription factor STAT3, ERK kinase, the synthesis of derivatives of the cyclooxygenase branch of metabolism of oxylipins and anandamide, and did not affect the formation of ROS. The study of the energy phenotype of cells showed that metformin activated glycolysis and inhibited mitochondrial respiration and oxidative phosphorylation, regardless of LPS stimulation and cell cultivation in high glucose concentration. Thus, it has been shown that metformin has anti-inflammatory effects, and its effect on the synthesis of cytokines, prostaglandins, and other lipid mediators can determine the beneficial effects of metformin in models of neuropathology. ; Установление взаимосвязи между воспалительными ответами и энергетическим метаболизмом важно для понимания биологии ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
Russian |
| العلاقة: |
https://repository.rudn.ru/records/article/record/86502Test/ |
| الإتاحة: |
https://repository.rudn.ru/records/article/record/86502Test/ |
| رقم الانضمام: |
edsbas.7C97010C |
| قاعدة البيانات: |
BASE |
