دورية أكاديمية
Long intergenic non-protein coding RNA 00707 regulates chondrocyte apoptosis and proliferation in osteoarthritis by serving as a sponge for microRNA-199-3p
| العنوان: | Long intergenic non-protein coding RNA 00707 regulates chondrocyte apoptosis and proliferation in osteoarthritis by serving as a sponge for microRNA-199-3p |
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| المؤلفون: | Yan Xu, Liang Duan, Shizhang Liu, Yuanyuan Yang, Zhi Qiao, Liang Shi |
| المصدر: | Bioengineered, Vol 13, Iss 4, Pp 11137-11145 (2022) |
| بيانات النشر: | Taylor & Francis Group |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | LINC00707, miR-199-3p, proliferation, apoptosis, osteoarthritis, Biotechnology, TP248.13-248.65 |
| الوصف: | It is known that long intergenic non-protein coding RNA 00707 (LINC00707) promotes lipopolysaccharide (LPS)-injury and microRNA-199-3p (miR-199-3p) regulates chondrocyte proliferation and apoptosis. Both processes participate in osteoarthritis (OA). We predicted that LINC00707 and miR-199-3p may interact with each other. Therefore, LINC00707 and miR-199-3p may interact with each other to participate in OA. In this study, the expression of LINC00707 and miR-199-3p in both OA and normal articular cartilage tissues was analyzed using RT-qPCR. The subcellular location of LINC00707 and its direct interaction with miR-199-3p were explored by nuclear fractionation assay, RNA pull-down assay and Luciferase reporter assay, respectively. The role of LINC00707 and miR-199-3p in regulating the expression of each other was analyzed using an overexpression assay, followed by RT-qPCR. The role of LINC00707 and miR-199-3p in regulating OA chondrocyte proliferation and apoptosis was analyzed by BrdU assay and cell apoptosis assay, respectively. OA tissues exhibited increased expression of LINC00707 and decreased expression of miR-199-3p. LINC00707 directly interacted with miR-199-3p in cytoplasm. However, LINC00707 and miR-199-3p overexpression failed to affect each other’s expression. LPS treatment increased LINC00707 expression and decreased miR-199-3p expression in OA chondrocyte. LINC00707 overexpression increased the apoptosis of OA chondrocytes induced by LPS and suppressed the proliferation of OA chondrocytes. Moreover, LINC00707 suppressed the role of miR-199-3p in enhancing cell proliferation and suppressing cell apoptosis. In conclusion, LINC00707 can be detected in cytoplasm and it may sponge miR-199-3p to regulate chondrocyte proliferation and apoptosis in OA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2165-5987 2165-5979 |
| العلاقة: | https://doaj.org/toc/2165-5979Test; https://doaj.org/toc/2165-5987Test; https://doaj.org/article/656d0194b88741cab5141730f9f5b4bdTest |
| DOI: | 10.1080/21655979.2022.2061287 |
| الإتاحة: | https://doi.org/10.1080/21655979.2022.2061287Test https://doaj.org/article/656d0194b88741cab5141730f9f5b4bdTest |
| رقم الانضمام: | edsbas.C7D08C1B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21655987 21655979 |
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| DOI: | 10.1080/21655979.2022.2061287 |