التفاصيل البيبلوغرافية
| العنوان: |
Biological control of oilseed rape Sclerotinia stem rot by Bacillus subtilis strain Em7. |
| المؤلفون: |
Gao, Xiaoning, Han, Qingmei, Chen, Yafei, Qin, Huqiang, Huang, Lili, Kang, Zhensheng |
| المصدر: |
Biocontrol Science & Technology; Jan2014, Vol. 24 Issue 1, p39-52, 14p |
| مصطلحات موضوعية: |
ENDOPHYTIC bacteria, OILSEEDS, RAPE (Plant), SCLEROTINIA diseases, BACILLUS subtilis, PHYSIOLOGICAL control systems, TRANSMISSION electron microscopy |
| مستخلص: |
In the present study, the endophytic bacteriumBacillus subtilisstrain Em7 (GU258545.1) was evaluated as a biological control agent forSclerotinia sclerotiorumon oilseed rape. In petri dish, strain Em7 not only strongly inhibited pathogen mycelium growth but also germination of sclerotia at concentrations between 109and 1011colony forming unit (CFU)·ml−1. Scanning electron microscopy and transmission electron microscopy studies revealed that in the presence of strain Em7, hyphae ofS. sclerotiorumshowed leakage and disintegration of hyphal cytoplasm. Furthermore, the strain Em7 showed a broad antifungal spectrum on mycelium growth of numerous important plant pathogenic fungi. Light microscopic observations revealed that strain Em7 caused morphological alterations including increased branching, swelling and collapse of cytoplasm. In the greenhouse, spray treatments of cell suspensions of strain Em7 (1×109CFU·ml−1) reduced leaf and stem rot incidence and severity in the seedling and blossom stage. The control efficacy was higher when strain Em7 cell suspension was applied one day prior to inoculation of the pathogen than after inoculation. Three-year field trials showed that two applications of strain Em7 cell suspension at blossom stage significantly reduced disease incidence and severity by 50–70%. There was no significant difference in control efficacy among treatments with strain Em7 cell suspension and the fungicides containing carbendazim or tebuconazole (P= 0.05). Thus, our results strongly suggest thatB. subtilisstrain Em7 is a promising biological control agent for control of oilseed rape Sclerotinia stem rot. [ABSTRACT FROM PUBLISHER] |
|
Copyright of Biocontrol Science & Technology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
