Lipid excess affects chaperone-mediated autophagy in hypothalamus
| العنوان: | Lipid excess affects chaperone-mediated autophagy in hypothalamus |
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| المؤلفون: | Christophe Magnan, Marcio Alberto Torsoni, Mariana Portovedo, M.P. Hakim, Marciane Milanski, Andressa Reginato, Josiane Miyamoto, Adriana Souza Torsoni, Letícia M. Ignacio-Souza, M. Campana, Raquel Franco Leal, Laís Angélica de Paula Simino, H. Le Stunff |
| المساهمون: | Universidade Estadual de Campinas (UNICAMP), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Universidade Estadual de Campinas = University of Campinas (UNICAMP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), ORANGE, Colette |
| المصدر: | Biochimie Biochimie, Elsevier, 2020, 176, pp.110-116. ⟨10.1016/j.biochi.2020.06.008⟩ Biochimie, 2020, 176, pp.110-116. ⟨10.1016/j.biochi.2020.06.008⟩ |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, DIET-INDUCED OBESITY, medicine.medical_specialty, [SDV]Life Sciences [q-bio], PATHOGENESIS, INHIBITION, Hypothalamus, Palmitic Acid, Alpha (ethology), Inflammation, Chaperone-Mediated Autophagy, Biology, Diet, High-Fat, Biochemistry, Cell Line, Palmitic acid, 03 medical and health sciences, chemistry.chemical_compound, Mice, Insulin resistance, Chaperone-mediated autophagy, BETA/NF-KAPPA-B, Internal medicine, medicine, Animals, Obesity, 2. Zero hunger, Neurons, INSULIN-RESISTANCE, 030102 biochemistry & molecular biology, Autophagy, General Medicine, medicine.disease, 3. Good health, [SDV] Life Sciences [q-bio], ALPHA, 030104 developmental biology, Endocrinology, chemistry, MACROAUTOPHAGY, Saturated fatty acid, medicine.symptom |
| الوصف: | International audience; Obesity is a major health problem worldwide. Overweight and obesity directly affect health-related quality of life and also have an important economic impact on healthcare systems. In experimental models, obesity leads to hypothalamic inflammation and loss of metabolic homeostasis. It is known that macroautophagy is decreased in the hypothalamus of obese mice but the role of chaperone-mediated autophagy is still unknown. In this study, we aimed to investigate the role of hypothalamic chaperone-mediated autophagy in response to high-fat diet and also the direct effect of palmitate on hypothalamic neurons. Mice received chow or high-fat diet for 3 days or 1 week. At the end of the experimental protocol, chaperone-mediated autophagy in hypothalamus was investigated, as well as cytokines expression. In other set of experiments, neuronal cell lines were treated with palmitic acid, a saturated fatty acid. We show that chaperone-mediated autophagy is differently regulated in response to high-fat diet intake for 3 days or 1 week. Also, when hypothalamic neurons are directly exposed to palmitate there is activation of chaperone-mediated autophagy. High-fat diet causes hypothalamic inflammation concomitantly to changes in the content of chaperone-mediated autophagy machinery. It remains to be studied the direct role of inflammation and lipids itself on the activation of chaperone-mediated autophagy in the hypothalamus in vivo and also the neuronal implications of chaperone-mediated autophagy inhibition in response to obesity. (c) 2020 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. |
| وصف الملف: | application/pdf |
| تدمد: | 1638-6183 0300-9084 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d024646a6080430b91d32ecf0b9e3a65Test https://pubmed.ncbi.nlm.nih.gov/32623049Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d024646a6080430b91d32ecf0b9e3a65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16386183 03009084 |
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