Synergistic and antibiofilm activity of the antimicrobial peptide P5 against carbapenem-resistant Pseudomonas aeruginosa
| العنوان: | Synergistic and antibiofilm activity of the antimicrobial peptide P5 against carbapenem-resistant Pseudomonas aeruginosa |
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| المؤلفون: | Liliana Semorile, Sónia Gonçalves, Mário R. Felício, Axel Hollmann, Melina María Belén Martínez, Nuno C. Santos, Patricia del Valle Maturana, Paulo Cesar Maffia |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.drug_class, 030106 microbiology, Antimicrobial peptides, Antibiotics, Biophysics, ANTIBIOFILM PEPTIDE, MEROPENEM, medicine.disease_cause, PSEUDOMONAS AERUGINOSA, Biochemistry, Bacterial cell structure, Microbiology, purl.org/becyt/ford/1 [https], Ciencias Biológicas, 03 medical and health sciences, SYNERGISTIC ACTIVITY, Drug Resistance, Bacterial, medicine, MTT assay, purl.org/becyt/ford/1.6 [https], ANTIMICROBIAL PEPTIDE, Pseudomonas aeruginosa, Chemistry, Biofilm, Cell Biology, Antimicrobial, Biofísica, Anti-Bacterial Agents, 030104 developmental biology, Carbapenems, Biofilms, BIOFILM, Bacterial outer membrane, CIENCIAS NATURALES Y EXACTAS, Antimicrobial Cationic Peptides |
| الوصف: | In the search for new antimicrobial molecules, antimicrobial peptides (AMPs) offer a viable alternative to conventional antibiotics, as they physically disrupt the bacterial membranes, leading to membrane disruption and eventually cell death. In particular, the group of linear α-helical cationic peptides has attracted increasing research and clinical interest. The AMP P5 has been previously designed as a cationic linear α-helical sequence, being its antimicrobial and hemolytic properties also evaluated. In this work, we analyzed the feasibility of using P5 against a carbapenem-resistant clinical isolate of Pseudomonas aeruginosa, one of the most common and risky pathogens in clinical practice. After antimicrobial activity confirmation in in vitro studies, synergistic activity of P5 with meropenem was evaluated, showing that P5 displayed significant synergistic activity in a time kill curve assay. The ability of P5 to permeabilize the outer membrane of P. aeruginosa can explain the obtained results. Finally, the antibiofilm activity was investigated by viability analysis (MTT assay), crystal violet and confocal imaging, with P5 displaying mild biofilm inhibition in the range of concentrations tested. Regarding biofilm disruption activity, P5 showed a higher efficacy, interfering with biofilm structure and promoting bacterial cell death. Atomic force microscope images further demonstrated the peptide potential in P. aeruginosa biofilm eradication, confirming the promising application of P5 in multi-resistant infections therapeutics. Fil: Martínez, Melina María Belén. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gonçalves, Sónia. Universidade Nova de Lisboa; Portugal Fil: Felício, Mário R.. Universidade Nova de Lisboa; Portugal Fil: Maturana, Patricia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina Fil: Santos, Nuno C.. Universidade Nova de Lisboa; Portugal Fil: Semorile, Liliana Carmen. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina Fil: Hollmann, Axel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Centro de Investigación en Biofísica Aplicada y Alimentos. - Universidad Nacional de Santiago del Estero. Centro de Investigación en Biofísica Aplicada y Alimentos; Argentina Fil: Maffia, Paulo Cesar. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| وصف الملف: | application/pdf |
| تدمد: | 1879-2642 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66d0acfe058efdfc459d1d2f31e74362Test https://pubmed.ncbi.nlm.nih.gov/31095945Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....66d0acfe058efdfc459d1d2f31e74362 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18792642 |
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