التفاصيل البيبلوغرافية
| العنوان: |
Intermittent hypoxia changes HIF-1α phosphorylation pattern in endothelial cells: Unravelling of a new PKA-dependent regulation of HIF-1α |
| المؤلفون: |
Olivier Feron, Carine Michiels, Sébastien Toffoli, Martine Raes |
| المصدر: |
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1773(10):1558-1571 |
| بيانات النشر: |
Elsevier BV, 2007. |
| سنة النشر: |
2007 |
| مصطلحات موضوعية: |
medicine.medical_specialty, Endothelium, HIF-1α, Biology, Cell Line, Enzyme activator, Internal medicine, medicine, Humans, PKA, Phosphorylation, Protein kinase B, Molecular Biology, Cell survival, Mitogen-Activated Protein Kinase 1, Sulfonamides, Mitogen-Activated Protein Kinase 3, Tumor, Intermittent hypoxia, Endothelial Cells, Cell Biology, Hypoxia (medical), Hypoxia-Inducible Factor 1, alpha Subunit, Isoquinolines, Cyclic AMP-Dependent Protein Kinases, Cell Hypoxia, Enzyme Activation, Oxygen, Endocrinology, medicine.anatomical_structure, Cell culture, Endothelium, Vascular, medicine.symptom, Proto-Oncogene Proteins c-akt, Neo-angiogenesis |
| الوصف: |
Vascularized tumors are exposed to intermittent hypoxia, that is, hypoxia followed by periods of reoxygenation. Abnormal structure and dysfunction of tumor blood vessels are responsible for these conditions. These repeated short periods of hypoxia concern tumor cells as well as endothelial cells. However, the effects of intermittent hypoxia are poorly understood. The aim of this study was to investigate the effects of intermittent hypoxia on endothelial cells and particularly on HIF-1α, a central actor in adaptive response to hypoxia. For that, endothelial cells were exposed to four repeated cycles of 1-h hypoxia followed by 30 min of reoxygenation. We showed that repeated cycles of hypoxia/reoxygenation induced a modification in HIF-lα phosphorylation pattern: a progressive increase in HIF-1α phosphorylated form was observed during the hypoxic periods. Activation of p42/p44, Akt and PKA was observed in parallel. PKA was shown to be involved in the phosphorylation of HIF-lα under intermittent hypoxia, while p42/p44 and Akt were not. As HIF-1 activity is often associated with enhanced cell survival, a better knowledge of the effects of intermittent hypoxia on endothelial cells and the highlight of particular mechanisms induced by intermittent hypoxia are essential to understand the behavior of endothelial cells during neo-angiogenesis. © 2007 Elsevier B.V. All rights reserved. |
| تدمد: |
0167-4889 |
| DOI: |
10.1016/j.bbamcr.2007.06.002 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::860b72a796b140c168934232053979e8Test |
| حقوق: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....860b72a796b140c168934232053979e8 |
| قاعدة البيانات: |
OpenAIRE |
