Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course
| العنوان: | Teaching bioprocess engineering to undergraduates: Multidisciplinary hands‐on training in a one‐week practical course |
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| المؤلفون: | Rudolf Hausmann, Marius Henkel, Anke Neumann, Christoph Syldatk, Michaela Zwick, Florian Oswald, Janina Beuker, Judit Willenbacher, Sandra Baumann, Martin Siemann-Herzberg |
| المصدر: | Biochemistry and Molecular Biology Education |
| بيانات النشر: | Wiley, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Class (computer programming), Process (engineering), Green Fluorescent Proteins, Biochemistry, Course (navigation), Laboratory Exercise, bioreactor, Engineering management, Bioreactors, Metabolic Engineering, Bioprocess engineering, Multidisciplinary approach, Engineering education, Escherichia coli, ComputingMilieux_COMPUTERSANDEDUCATION, Ultraviolet light, Humans, Genetic Engineering, Molecular Biology, bioprocess engineering, biotechnology, Interdisciplinarity |
| الوصف: | Bioprocess engineering is a highly interdisciplinary field of study which is strongly benefited by practical courses where students can actively experience the interconnection between biology, engineering, and physical sciences. This work describes a lab course developed for 2nd year undergraduate students of bioprocess engineering and related disciplines, where students are challenged with a real‐life bioprocess‐engineering application, the production of recombinant protein in a fed‐batch process. The lab course was designed to introduce students to the subject of operating and supervising an experiment in a bioreactor, along with the analysis of collected data and a final critical evaluation of the experiment. To provide visual feedback of the experimental outcome, the organism used during class was Escherichia coli which carried a plasmid to recombinantly produce enhanced green fluorescent protein (eGFP) upon induction. This can easily be visualized in both the bioreactor and samples by using ultraviolet light. The lab course is performed with bioreactors of the simplest design, and is therefore highly flexible, robust and easy to reproduce. As part of this work the implementation and framework, the results, the evaluation and assessment of student learning combined with opinion surveys are presented, which provides a basis for instructors intending to implement a similar lab course at their respective institution. © 2015 by the International Union of Biochemistry and Molecular Biology, 43(3):189–202, 2015. |
| تدمد: | 1539-3429 1470-8175 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95e6966c03b1405048f2167a05ab25cbTest https://doi.org/10.1002/bmb.20860Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....95e6966c03b1405048f2167a05ab25cb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15393429 14708175 |
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