A sensitive fluorographic method for the detection of nopaline and octopine synthase activities in Brassica crown-gall tissues
| العنوان: | A sensitive fluorographic method for the detection of nopaline and octopine synthase activities in Brassica crown-gall tissues |
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| المؤلفون: | Brian Miki, Margaret Haffner, Larry A. Holbrook |
| المصدر: | Biochemistry and Cell Biology. 64:126-132 |
| بيانات النشر: | Canadian Science Publishing, 1986. |
| سنة النشر: | 1986 |
| مصطلحات موضوعية: | Octopine, ATP synthase, biology, Arginine, Crown (botany), Brassica, Opine, Cell Biology, biology.organism_classification, Biochemistry, chemistry.chemical_compound, chemistry, biology.protein, Gall, Nopaline, Molecular Biology |
| الوصف: | L-[guanido-14C]Arginine has been used as a precursor for nopaline and octopine syntheses in the in vitro assay for opine synthase activities. Fluorographic detection of [14C]nopaline and [14C]octopine was optimized following their separation from other labelled compounds by paper electrophoresis. Compared with detection by phenanthrenequinone, fluorography was more specific for the guanidine compounds and severalfold more sensitive. Alternate precursors such as α-[14C]ketoglutarate or [14C]pyruvate were poor substitutes for [14C]arginine because they generated compounds that comigrated with opines. Four nonopine radioactive spots recurred in the in vitro assays. These have not been identified but have been distinguished from both octopine and nopaline. This technique has provided clear evidence for transformation of Brassica species by Ti plasmids of Agrobacterium tumefaciens and will be valuable in the analysis of extremely small tissue samples. |
| تدمد: | 1208-6002 0829-8211 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::ec07a9327c80bcaf83701cf8f0b9e5d5Test https://doi.org/10.1139/o86-020Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........ec07a9327c80bcaf83701cf8f0b9e5d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 12086002 08298211 |
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