التفاصيل البيبلوغرافية
| العنوان: |
Purification and Some Properties of Lysostaphin, a Glycylglycine Endopeptidase from the Culture Liquid of Staphylococcus simulans biovar staphylolyticus. |
| المؤلفون: |
Fedorov, T. V., Surovtsev, V. I., Pletnev, V. Z., Borozdina, M. A., Gusev, V. V. |
| المصدر: |
Biochemistry (00062979); Jan2003, Vol. 68 Issue 1, p50-53, 4p |
| مصطلحات موضوعية: |
CHEMICAL purification, ENDOPEPTIDASES, ION exchange chromatography, ULTRAFILTRATION, ENZYME analysis, CHEMICAL processes, BIOCHEMISTRY |
| مستخلص: |
This work presents a method for purification of lysostaphin, a glycylglycine endopeptidase, from the culture liquid of S. simulans biovar staphylolyticus to homogeneity in a few steps. The method includes ultrafiltration and ion-exchange and hydrophobic chromatographies. The enzyme was isolated in preparative amounts with the yield of 51%. Some physical and chemical properties of the enzyme are described. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
