Variations in Antigen−Antibody Association Kinetics as a Function of pH and Salt Concentration: A QSAR and Molecular Modeling Study
| العنوان: | Variations in Antigen−Antibody Association Kinetics as a Function of pH and Salt Concentration: A QSAR and Molecular Modeling Study |
|---|---|
| المؤلفون: | Danièle Altschuh, Laurence Choulier, Erwin De Genst, Virginie Lafont, Annick Dejaegere |
| المساهمون: | Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Cellular and Molecular Immunology, Vrije Universiteit Brussel, Klotz, Evelyne |
| المصدر: | Biochemistry Biochemistry, American Chemical Society, 2005, 44 (44), pp.14409-14418. ⟨10.1021/bi050986v⟩ Biochemistry, American Chemical Society, 2005, 44, pp.14409-18 Biochemistry, American Chemical Society, 2005, 44 (44), pp.14409-18. ⟨10.1021/bi050986v⟩ |
| بيانات النشر: | American Chemical Society (ACS), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Models, Molecular, MESH: Hydrogen-Ion Concentration, Molecular model, [SDV]Life Sciences [q-bio], Molecular binding, Quantitative Structure-Activity Relationship, Antigen-Antibody Complex, 01 natural sciences, Biochemistry, Models, MESH: Animals, Salts/*chemistry, Non-U.S. Gov't, ComputingMilieux_MISCELLANEOUS, MESH: Quantitative Structure-Activity Relationship, 0303 health sciences, Antibodies/*chemistry/genetics/metabolism, Chemistry, Antigens/*chemistry/genetics/metabolism, Temperature, MESH: Antigen-Antibody Complex, Hydrogen-Ion Concentration, MESH: Temperature, Receptor–ligand kinetics, MESH: Salts, MESH: Antigens, Titration, MESH: Models, Molecular, Muramidase/chemistry/genetics/metabolism, Protein Binding, Quantitative structure–activity relationship, Kinetics, Thermodynamics, Research Support, 010402 general chemistry, MESH: Multivariate Analysis, Antibodies, 03 medical and health sciences, MESH: Protein Binding, Animals, Antigens, 030304 developmental biology, MESH: Antibodies, Solvation, Molecular, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 0104 chemical sciences, Crystallography, Ionic strength, MESH: Muramidase, Multivariate Analysis, Muramidase, Salts, Antigen-Antibody Complex/*chemistry |
| الوصف: | 0006-2960 (Print) Journal Article; The relationship between three environmental factors (ionic strength, pH, and temperature) and antigen-antibody binding kinetics was investigated using QSAR (quantitative structure-activity relationship) and molecular modeling approaches. The interaction used for this analysis is that between the camel antibody fragment cAbLys3 and lysozyme. Binding kinetics were measured using a Biacore 2000 instrument, at NaCl concentrations between 50 and 500 mM, at pH's between 5 and 10, and at temperatures between 15 and 30 degrees C, according to multivariate experimental designs. Variations in kinetic on- and off-rate parameters were up to 400- and 16-fold, respectively. Mathematical models that relate log k(on) to experimental conditions were developed. They indicated an influence of all three factors, with a clear dependency between pH and NaCl concentration for their effect on k(on). These models were able to predict on-rate parameters under new experimental conditions. Titration calculations using continuum electrostatics were performed on the crystallographic structures of the isolated and bound proteins to gain structural insight for the on-rate enhancement observed at pH |
| تدمد: | 1520-4995 0006-2960 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ba78f2b5992dd255cd125756e2ba4f6Test https://doi.org/10.1021/bi050986vTest |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....4ba78f2b5992dd255cd125756e2ba4f6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204995 00062960 |
|---|