Gene family of male-specific testosterone 16.alpha.-hydroxylase (C-P-45016.alpha.) in mouse liver: cDNA sequences, neonatal imprinting, and reversible regulation by androgen
| العنوان: | Gene family of male-specific testosterone 16.alpha.-hydroxylase (C-P-45016.alpha.) in mouse liver: cDNA sequences, neonatal imprinting, and reversible regulation by androgen |
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| المؤلفون: | Kaname Kawajiri, Garry Wong, Masahiko Negishi |
| المصدر: | Biochemistry. 26:8683-8690 |
| بيانات النشر: | American Chemical Society (ACS), 1987. |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Male, Base pair, Molecular Sequence Data, Mice, Inbred Strains, Biology, Biochemistry, Isozyme, Mice, chemistry.chemical_compound, Cytochrome P-450 Enzyme System, Species Specificity, Complementary DNA, Animals, Coding region, Amino Acid Sequence, Cloning, Molecular, Cytochrome P450 Family 2, Gene, Mice, Inbred BALB C, Base Sequence, Protein primary structure, Nucleic acid sequence, Nucleic Acid Hybridization, DNA, DNA Restriction Enzymes, Molecular biology, Isoenzymes, Mice, Inbred C57BL, Genes, Liver, Steroid 16-alpha-Hydroxylase, chemistry, Steroid Hydroxylases, Aryl Hydrocarbon Hydroxylases |
| الوصف: | The cDNA clone p16..cap alpha..-1 for the male-specific isozyme (C-P-450/sub 16..cap alpha../)/sup 1/ of testosterone 16..cap alpha..-hydroxylase in livers of 129/J mice and two additional full-length cDNAs overlapping with p16..cap alpha..-1 (p16..cap alpha..-2 and p16..cap alpha..-16) were sequenced. p16..cap alpha..-2 contained a single open reading frame of 1512 nucleotides, consisting of 71 base pairs of the 5'-noncoding region and 63 base pairs of the 3'-noncoding region with an additional poly(A) tract. From this DNA sequence, C-P-450/sub 16..cap alpha../ was deduced to contain 504 amino acids with a calculated molecular mass of 56,948 daltons. p16..cap alpha..-1 showed a nucleotide sequence identical with that of p16..cap alpha..-2 but lacked nine amino acid residues from the N-terminus. Another cDNA clone, p16..cap alpha..-16, also exhibited the same coding sequence with the exception of a 142 base pair deletion spanning from nucleotide 853 to nucleotide 994 of p16..cap alpha..-2. The putative poly(A) addition signal AATAAA is present for all three clones, but there are polymorphisms in the start sites of polyadenylation. Amino acid alignment with P-450b, P-450 M1, and P-450c revealed that P-450/sub 16..cap alpha../ is 39.8%, 40.7%, and 31.5% homologous, respectively. Five highly homologous genes were selected from a genomic DNA library of BALB/cJmore » mice by plaque hybridization to the cDNA and characterized. The results indicated that p16..cap alpha..-1 recognizes at least two mRNAs in terms of their regulation by androgen; one is reversibly regulated and the other is neonatally imprinted in expression in adult livers. The two differentially regulated C-P-450/sub 16..cap alpha../ dependent testosterone 16..cap alpha..-hydroxylase activities in the liver microsomes from these mice were also detected and associated with the hybridizable mRNA levels. A hybridization of the specific oligonucleotide probe with the poly(A) + RNA concluded that p16..cap alpha..-1 and p16..cap alpha..-2 encode C-P-450/sub 16..cap alpha../, which is reversibly regulated.« less |
| تدمد: | 1520-4995 0006-2960 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0317199829631617bac21d44436b9b0fTest https://doi.org/10.1021/bi00400a029Test |
| رقم الانضمام: | edsair.doi.dedup.....0317199829631617bac21d44436b9b0f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15204995 00062960 |
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