Measurement of the oxidation-reduction potentials for one-electron and two-electron reduction of electron-transfer flavoprotein from pig liver
العنوان: | Measurement of the oxidation-reduction potentials for one-electron and two-electron reduction of electron-transfer flavoprotein from pig liver |
---|---|
المؤلفون: | Brian G. Fox, Marian T. Stankovich, M Husain |
المصدر: | Biochemical Journal. 219:1043-1047 |
بيانات النشر: | Portland Press Ltd., 1984. |
سنة النشر: | 1984 |
مصطلحات موضوعية: | Semiquinone, Electron-Transferring Flavoproteins, Swine, Potentiometric titration, Flavoprotein, Mitochondria, Liver, Oxidative phosphorylation, Photochemistry, Biochemistry, Electron-transferring flavoprotein, Electron Transport, Electron transfer, chemistry.chemical_compound, Animals, Molecular Biology, Flavoproteins, biology, Hydroquinone, Chemistry, Quinones, Cell Biology, Electron transport chain, Potentiometry, biology.protein, Oxidation-Reduction, Research Article |
الوصف: | Potentiometric titrations of pig liver electron-transfer flavoprotein (ETF) were performed at pH 7.5 and 4 degrees C, both in the reductive and oxidative directions. Reduction of ETF to the hydroquinone form required a total of two reducing equivalents/mol of ETF with the formation of sub-stoichiometric amounts of anionic semiquinone as an intermediate. The oxidation-reduction potentials for the two one-electron couples, oxidized ETF/ETF semiquinone and ETF semiquinone/fully reduced ETF, are +4 mV and -50 mV respectively. The overall midpoint potential for the two-electron couple (oxidized ETF/fully reduced ETF) is -23 mV. |
تدمد: | 1470-8728 0264-6021 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0130727320f91a3ee6b8ac6a51f3d783Test https://doi.org/10.1042/bj2191043Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....0130727320f91a3ee6b8ac6a51f3d783 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14708728 02646021 |
---|