Branched-chain-amino-acid biosynthesis in plants: molecular cloning and characterization of the gene encoding acetohydroxy acid isomeroreductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress)
| العنوان: | Branched-chain-amino-acid biosynthesis in plants: molecular cloning and characterization of the gene encoding acetohydroxy acid isomeroreductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress) |
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| المؤلفون: | Roland Douce, Renaud Dumas, Gilles Curien, R T DeRose |
| المصدر: | Biochemical Journal. 294:821-828 |
| بيانات النشر: | Portland Press Ltd., 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | RNA Splicing, Ketol-Acid Reductoisomerase, Molecular Sequence Data, Restriction Mapping, Branched-chain amino acid, Arabidopsis, Molecular cloning, Biology, Genes, Plant, Biochemistry, chemistry.chemical_compound, Complementary DNA, Coding region, Arabidopsis thaliana, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Gene, Genetics, Base Sequence, Nucleic acid sequence, Hydrogen Bonding, Cell Biology, biology.organism_classification, Introns, Alcohol Oxidoreductases, genomic DNA, Oligodeoxyribonucleotides, chemistry, Nucleic Acid Conformation, Sequence Alignment, Research Article |
| الوصف: | Towards the goal of gaining a better understanding of the molecular mechanisms controlling branched-chain-amino-acid biosynthesis in plants, we have isolated, sequenced and characterized a gene encoding acetohydroxy acid isomero-reductase (ketol-acid reductoisomerase) from Arabidopsis thaliana (thale cress). Comparison between the acetohydroxy acid isomeroreductase cDNA and the genomic sequence has allowed us to determine the exon structure of the coding region. The isolated acetohydroxy acid isomeroreductase gene is distributed over approx. 4.5 kbp and contains nine introns (79-347 bp). The transcriptional start site was found to be 52 bp upstream of the translational initiation site. Southern-blot analysis of A. thaliana genomic DNA shows that the acetohydroxy acid isomeroreductase is encoded by a single-copy gene. |
| تدمد: | 1470-8728 0264-6021 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89d8618c5380e4d49c47940d03f1af22Test https://doi.org/10.1042/bj2940821Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....89d8618c5380e4d49c47940d03f1af22 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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