التفاصيل البيبلوغرافية
| العنوان: |
Altered microRNAs in STHdhQ111 /HdhQ111 cells: miR-146a targets TBP |
| المؤلفون: |
Sinha, Mithun1, Ghose, Jayeeta2, Das, Eashita2, Bhattarcharyya, Nitai P.1,2 nitai_sinp@yahoo.com |
| المصدر: |
Biochemical & Biophysical Research Communications. Jun2010, Vol. 396 Issue 3, p742-747. 6p. |
| مصطلحات موضوعية: |
*ANTISENSE RNA, *CARRIER proteins, *GENE targeting, *HUNTINGTON disease, *GENETIC regulation, *LUCIFERASES, *BIOLOGICAL assay, *GENETICS |
| مستخلص: |
Abstract: We studied expression of 90 miRNAs in STHdhQ111 /HdhQ111 cells, a model for Huntington’s disease and compared with that obtained in STHdhQ7 /HdhQ7 cells. Fifteen miRNAs were down regulated and 12 miRNAs were up regulated more than 2-fold. Such changes were statistically significant. One hundred and forty-two genes are experimentally known targets of these altered miRNAs. It has been predicted that miR-146a may target Tata Binding Protein (TBP). Using luciferase reporter assays with 3′-UTRs of TBP, over-expression and inhibition of miR-146a, we showed that miR-146a targets TBP. Regulation of TBP by miR-146a may contribute to HD pathogenesis. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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