التفاصيل البيبلوغرافية
| العنوان: |
Biochemical and structural characterization of a keratin-degrading M32 carboxypeptidase from Fervidobacterium islandicum AW-1. |
| المؤلفون: |
Lee, Yong-Jik1, Dhanasingh, Immanuel2, Ahn, Jin-Soo1, Jin, Hyeon-Su1, Choi, Jin Myung2, Lee, Sung Haeng2 sunglee@chosun.ac.kr, Lee, Dong-Woo1 leehicam@knu.ac.kr |
| المصدر: |
Biochemical & Biophysical Research Communications. Dec2015, Vol. 468 Issue 4, p927-933. 7p. |
| مصطلحات موضوعية: |
*KERATIN, *CARBOXYPEPTIDASES, *THERMOPHILIC bacteria, *GENETIC code, *AMINO acid sequence, *GENETIC overexpression |
| مستخلص: |
Comparative genomics of the keratin-degrading extremophilic eubacterium Fervidobacterium islandicum AW-1 and the closely related Fervidobacterium nodosum with no keratinolytic activity suggested that the FIAW1_1600 gene encoding a carboxypeptidase (CP) plays an important role in keratin degradation. The presumptive 489 amino acid sequence of the gene showed a conserved HEXXH motif with low levels of sequence identity (<38%) to reported thermostable M32 CPs. To identify its functional role, the FIAW1_1600 gene was overexpressed in Escherichia coli , and the recombinant enzyme was purified and characterized in detail. F. islandicum AW-1 CP (FisCP) formed a homodimer with a molecular mass of 107 kDa, and its apoenzyme exhibited maximal activity at 80 °C and pH 7.0 in the presence of Co 2+ . This metalloenzyme mainly cleaved the C-termini of peptides with a basic amino acid sequence. The crystal structure of FisCP at 2.2 Å resolution showed high levels of structural similarities (root-mean-square deviations of <1.7 Å) to those of other M32 CP homologs. Remarkably, the enzyme significantly enhanced the degradation of native chicken feathers. This study suggests that FisCP, a keratinolytic member of the thermostable M32 CP family, plays an important role in keratin degradation for cellular metabolism in F. islandicum AW-1. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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