دورية أكاديمية
Untargeted Mass Spectrometry Approach to Study SARS-CoV-2 Proteins in Human Plasma and Saliva Proteome
| العنوان: | Untargeted Mass Spectrometry Approach to Study SARS-CoV-2 Proteins in Human Plasma and Saliva Proteome |
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| المؤلفون: | Lisa Pagani, Clizia Chinello, Allia Mahajneh, Francesca Clerici, Lucrezia Criscuolo, Andrea Favalli, Paola Gruarin, Renata Grifantini, Alessandra Bandera, Andrea Lombardi, Riccardo Ungaro, Antonio Muscatello, Francesco Blasi, Andrea Gori, Fulvio Magni |
| المصدر: | BioChem, Vol 2, Iss 1, Pp 64-82 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Therapeutics. Pharmacology LCC:Biochemistry |
| مصطلحات موضوعية: | COVID-19, saliva, plasma, proteomics, biomarker, Therapeutics. Pharmacology, RM1-950, Biochemistry, QD415-436 |
| الوصف: | Since the start of the COVID-19 outbreak, more than four million people have died of this disease. Given its ability to provide a precise response, mass spectrometry-based proteomics could represent a useful tool to study this pathology. To this end, an untargeted nLC-ESI-MS/MS-based method to characterise SARS-CoV-2 proteins, including possible variants, and investigate human saliva and plasma proteome in a single analysis was developed for further application in patients. Four SARS-CoV-2 recombinant proteins, three (S1–S2–RBD) belonging to the spike glycoprotein (S) and one corresponding to the nucleoprotein (N), were prepared and analysed with nLC-UHRTOF by injecting decreasing amounts to establish the limit of detection (LOD) of the method. This was determined as 10 pg for all the components of the S protein and for N (71 amol and 213 amol, respectively). Various viral inactivation strategies plus deglycosylation and digestion approaches were then tested in saliva and plasma spiked with different quantities of SARS-CoV-2 recombinant proteins. The limit of characterisation (LOC) in saliva for the N and S proteins was observed at 100 pg (coverage of 20% and 3%, respectively); instead, in plasma, it was 33 pg for N and 330 pg for the S protein, with a coverage of 4% for both. About 300 and 800 human proteins were identified in plasma and saliva, respectively, including several key effectors and pathways that are known to be altered in COVID-19 patients. In conclusion, this approach allows SARS-CoV-2 proteins and the human proteome to be simultaneously explored, both for plasma and saliva, showing a high relevant potential for retrospective studies aimed at investigating possible virus variants and for patient stratification. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2673-6411 |
| العلاقة: | https://www.mdpi.com/2673-6411/2/1/5Test; https://doaj.org/toc/2673-6411Test |
| DOI: | 10.3390/biochem2010005 |
| الوصول الحر: | https://doaj.org/article/9cb098c6d25d4b23bd783031d8851d6cTest |
| رقم الانضمام: | edsdoj.9cb098c6d25d4b23bd783031d8851d6c |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 26736411 |
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| DOI: | 10.3390/biochem2010005 |