Development and evaluation of an ultrasensitive free VEGF-A immunoassay for analysis of human aqueous humor
| العنوان: | Development and evaluation of an ultrasensitive free VEGF-A immunoassay for analysis of human aqueous humor |
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| المؤلفون: | Thomas O. Joos, Uwe Wessels, Everson Nogoceke, Astrid Reiser, Jens C. Göpfert, Anne Heine, Corinne Petit-Frere, Annelie Pohle, Kay-Gunnar Stubenrauch, Viviana A. Carcamo Yañez |
| المصدر: | Bioanalysis. 11:875-886 |
| بيانات النشر: | Future Science Ltd, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Vascular Endothelial Growth Factor A, medicine.medical_specialty, VEGF receptors, Clinical Biochemistry, Aqueous humor, 030226 pharmacology & pharmacy, 01 natural sciences, Macular Edema, Analytical Chemistry, Aqueous Humor, Diabetes Complications, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Limit of Detection, Ophthalmology, Humans, Medicine, General Pharmacology, Toxicology and Pharmaceutics, Immunoassay, biology, medicine.diagnostic_test, business.industry, 010401 analytical chemistry, Retinal, General Medicine, Diabetic retinopathy, Macular degeneration, medicine.disease, 0104 chemical sciences, Medical Laboratory Technology, chemistry, Calibration, biology.protein, Biomarker (medicine), business |
| الوصف: | Aim: Novel bifunctional VEGF-A neutralizing therapies are being developed for the treatment of retinal vascular diseases such as age-related macular degeneration and diabetic retinopathy. In developing new therapeutic drugs, only small aqueous humor sample volumes are available for analyzing several parameters. Highly sensitive detection methods must be applied in analyzing VEGF-A levels in ocular fluids in order to demonstrate VEGF-A suppression following drug administration. Experimental: A highly sensitive immunoassay for VEGF-A was developed on the single molecule array (Simoa) platform, and validated before being used for the analysis of clinical aqueous humor samples from patients treated with anti-VEGF-A therapeutics. Results: This highly sensitive immunoassay allows the detection of baseline VEGF-A levels and suppression effects after drug administration, even in sample volumes as low as 12 μl. Conclusion: The Simoa VEGF-A assay is a valuable tool for the reliable monitoring of VEGF-A suppression after intravitreal administration of anti-VEGF-A drugs. |
| تدمد: | 1757-6199 1757-6180 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7910c0551f64f45370d8805d2c5ecc2aTest https://doi.org/10.4155/bio-2019-0044Test |
| رقم الانضمام: | edsair.doi.dedup.....7910c0551f64f45370d8805d2c5ecc2a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17576199 17576180 |
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