In the peripheral nervous system, Schwann cells are the primary type of glia; their in vitro differentiation and dedifferentiation system has not been described in detail in the literature. Thus, an in vitro differentiation and dedifferentiation system of rat Schwann cells is described in this protocol. These cultures and systems may be used to investigate the morphological and biochemical effects of drug interventions or lentivirus-mediated gene transfer on Schwann cells during differentiation or dedifferentiation.Graphical abstract
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