SarkoSpin: A Technique for Biochemical Isolation and Characterization of Pathological TDP-43 Aggregates
| العنوان: | SarkoSpin: A Technique for Biochemical Isolation and Characterization of Pathological TDP-43 Aggregates |
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| المؤلفون: | Magdalini Polymenidou, Florent Laferrière, Manuela Pérez-Berlanga |
| المساهمون: | University of Zurich, Universität Zürich [Zürich] = University of Zurich (UZH) |
| المصدر: | Bio-protocol Bio-protocol, Bio-protocol LCC, 2019, 9 (22), ⟨10.21769/BioProtoc.3424⟩ Bio Protoc |
| بيانات النشر: | Bio-Protocol, LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Strategy and Management, 610 Medicine & health, Biology, Industrial and Manufacturing Engineering, 03 medical and health sciences, 0302 clinical medicine, mental disorders, Methods Article, medicine, Amyotrophic lateral sclerosis, Pathological, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Detergent solubilization, 0303 health sciences, Mechanical Engineering, Neurodegeneration, Metals and Alloys, nutritional and metabolic diseases, Frontotemporal lobar degeneration, medicine.disease, nervous system diseases, 3. Good health, Solubilization, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], 11493 Department of Quantitative Biomedicine, 030217 neurology & neurosurgery |
| الوصف: | TDP-43 is the main aggregating protein in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Aggregated TDP-43 is resistant to diverse detergent solubilization, yet physiological TDP-43 and other abundant proteins commonly co-purify with pathological TDP-43. This mixed isolation has precluded the elucidation of the biochemical and structural features of the pathological TDP-43 and its role in disease. Here we describe SarkoSpin, a method for the isolation of pure pathological TDP-43 from patient autopsy brain by sample solubilization with Sarkosyl after nuclease treatment. This purification, which is also applicable to cell culture material, permits the study of biochemical properties of exclusively pathological TDP-43, allowing for the first time the determination of their link to the clinical presentation of FTLD. This method opens up a path for the study of pathological TDP-43 at the molecular and structural level in the heterogeneous spectrum of ALS and FTLD cases. |
| وصف الملف: | Perez-Berlanga_etal_Bio-protocol3424.pdf - application/pdf |
| تدمد: | 2331-8325 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52daedf2733ad0aa176d4237c4a4ada3Test https://doi.org/10.21769/bioprotoc.3424Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....52daedf2733ad0aa176d4237c4a4ada3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23318325 |
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