Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy
| العنوان: | Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy |
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| المؤلفون: | Boris N. Khlebtsov, Nikolai G. Khlebtsov, Timofey Pylaev, Andrey M. Burov |
| المصدر: | Beilstein Journal of Nanotechnology, Vol 10, Iss 1, Pp 794-803 (2019) Beilstein Journal of Nanotechnology |
| بيانات النشر: | Beilstein-Institut, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Materials science, General Physics and Astronomy, Nanoparticle, Nanotechnology, Au nanorods, targeted phototherapy, 02 engineering and technology, Polyethylene glycol, 010402 general chemistry, folate, lcsh:Chemical technology, 01 natural sciences, lcsh:Technology, Full Research Paper, HeLa, chemistry.chemical_compound, fluorescent bioimaging, Fluorescence microscope, General Materials Science, lcsh:TP1-1185, Electrical and Electronic Engineering, lcsh:Science, polydopamine, Nanocomposite, biology, lcsh:T, Photothermal therapy, 021001 nanoscience & nanotechnology, biology.organism_classification, lcsh:QC1-999, 0104 chemical sciences, Nanoscience, chemistry, Cancer cell, Nanorod, lcsh:Q, cancer theranostics, 0210 nano-technology, lcsh:Physics |
| الوصف: | Au nanorods (AuNRs) have attracted a great interest as a platform for constructing various composite core/shell nanoparticles for theranostics applications. However, the development of robust methods for coating AuNRs with a biocompatible shell of high loading capacity and with functional groups still remains challenging. Here, we coated AuNRs with a polydopamine (PDA) shell and functionalized AuNR-PDA particles with folic acid and rhodamine 123 (R123) to fabricate AuNR-PDA-R123-folate nanocomposites. To the best of our knowledge, such AuNR-PDA-based composites combining fluorescent imaging and plasmonic phothothermal abilities have not been reported previously. The multifunctional nanoparticles were stable in cell buffer, nontoxic and suitable for targeted fluorescent imaging and photothermal therapy of cancer cells. We demonstrate the enhanced accumulation of folate-functionalized nanoparticles in folate-positive HeLa cells in contrast to the folate-negative HEK 293 cells using fluorescent microscopy. The replacement of folic acid with polyethylene glycol (PEG) leads to a decrease in nanoparticle uptake by both folate-positive and folate-negative cells. We performed NIR light-mediated targeted phototherapy using AuNR-PDA-R123-folate and obtained a remarkable cancer cell killing efficiency in vitro in comparison with only weak-efficient nontargeted PEGylated nanoparticles. Our work illustrates that AuNR-PDA could be a promising nanoplatform for multifunctional tumor theranostics in the future. |
| اللغة: | English |
| تدمد: | 2190-4286 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::64fd9fcb75a4647bfa775bc6574d40bbTest https://doaj.org/article/2548a3dff22d4a3c9a7a40c399c69926Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....64fd9fcb75a4647bfa775bc6574d40bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21904286 |
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