دورية أكاديمية
Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells
| العنوان: | Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells |
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| المؤلفون: | Eloïse Equy, Jordana Hirtzel, Sophie Hellé, Béatrice Heurtault, Eric Mathieu, Morgane Rabineau, Vincent Ball, Lydie Ploux |
| المصدر: | Beilstein Journal of Nanotechnology, Vol 14, Iss 1, Pp 1208-1224 (2023) |
| بيانات النشر: | Beilstein-Institut, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Technology LCC:Chemical technology LCC:Science LCC:Physics |
| مصطلحات موضوعية: | accumulation, albumin, antibacterial, escherichia coli, fluorescence, nanoparticles, penetration, polydopamine, Technology, Chemical technology, TP1-1185, Science, Physics, QC1-999 |
| الوصف: | Inspired by the eumelanin aggregates in human skin, polydopamine nanoparticles (PDA NPs) are promising nanovectors for biomedical applications, especially because of their biocompatibility. We synthesized and characterized fluorescent PDA NPs of 10–25 nm diameter based on a protein containing a lysine–glutamate diad (bovine serum albumin, BSA) and determined whether they can penetrate and accumulate in bacterial cells to serve as a marker or drug nanocarrier. Three fluorescent PDA NPs were designed to allow for tracking in three different wavelength ranges by oxidizing BSA/PDA NPs (Ox-BSA/PDA NPs) or labelling with fluorescein 5-isothiocyanate (FITC-BSA/PDA NPs) or rhodamine B isothiocyanate (RhBITC-BSA/PDA NPs). FITC-BSA/PDA NPs and RhBITC-BSA/PDA NPs penetrated and accumulated in both cell wall and inner compartments of Escherichia coli (E. coli) cells. The fluorescence signals were diffuse or displayed aggregate-like patterns with both labelled NPs and free dyes. RhBITC-BSA/PDA NPs led to the most intense fluorescence in cells. Penetration and accumulation of NPs was not accompanied by a bactericidal or inhibitory effect of growth as demonstrated with the Gram-negative E. coli species and confirmed with a Gram-positive bacterial species (Staphylococcus aureus). Altogether, these results allow us to envisage the use of labelled BSA/PDA NPs to track bacteria and carry drugs in the core of bacterial cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2190-4286 |
| العلاقة: | https://doaj.org/toc/2190-4286Test |
| DOI: | 10.3762/bjnano.14.100 |
| الوصول الحر: | https://doaj.org/article/bd2618ca21354bb4abf693465dff2558Test |
| رقم الانضمام: | edsdoj.bd2618ca21354bb4abf693465dff2558 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21904286 |
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| DOI: | 10.3762/bjnano.14.100 |