التفاصيل البيبلوغرافية
| العنوان: |
Yeast growth in raffinose results in resistance to acetic-acid induced programmed cell death mostly due to the activation of the mitochondrial retrograde pathway. |
| المؤلفون: |
Guaragnella, Nicoletta1, Ždralević, Maša1, Lattanzio, Paolo1, Marzulli, Domenico1, Pracheil, Tammy2, Liu, Zhengchang2, Passarella, Salvatore3, Marra, Ersilia1, Giannattasio, Sergio1 s.giannattasio@ibbe.cnr.it |
| المصدر: |
BBA - Molecular Cell Research. Dec2013, Vol. 1833 Issue 12, p2765-2774. 10p. |
| مصطلحات موضوعية: |
*FUNGAL growth, *RAFFINOSE, *ACETIC acid, *APOPTOSIS, *MITOCHONDRIA, *CITRATE synthase |
| مستخلص: |
Abstract: In order to investigate whether and how a modification of mitochondrial metabolism can affect yeast sensitivity to programmed cell death (PCD) induced by acetic acid (AA-PCD), yeast cells were grown on raffinose, as a sole carbon source, which, differently from glucose, favours mitochondrial respiration. We found that, differently from glucose-grown cells, raffinose-grown cells were mostly resistant to AA-PCD and that this was due to the activation of mitochondrial retrograde (RTG) response, which increased with time, as revealed by the up-regulation of the peroxisomal isoform of citrate synthase and isocitrate dehydrogenase isoform 1, RTG pathway target genes. Accordingly, the deletion of RTG2 and RTG3, a positive regulator and a transcription factor of the RTG pathway, resulted in AA-PCD, as shown by TUNEL assay. Neither deletion in raffinose-grown cells of HAP4, encoding the positive regulatory subunit of the Hap2,3,4,5 complex nor constitutive activation of the RTG pathway in glucose-grown cells due to deletion of MKS1, a negative regulator of RTG pathway, had effect on yeast AA-PCD. The RTG pathway was found to be activated in yeast cells containing mitochondria, in which membrane potential was measured, capable to consume oxygen in a manner stimulated by the uncoupler CCCP and inhibited by the respiratory chain inhibitor antimycin A. AA-PCD resistance in raffinose-grown cells occurs with a decrease in both ROS production and cytochrome c release as compared to glucose-grown cells en route to AA-PCD. [Copyright &y& Elsevier] |
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