التفاصيل البيبلوغرافية
| العنوان: |
SET7/9 interacts and methylates the ribosomal protein, eL42 and regulates protein synthesis. |
| المؤلفون: |
Mahesh, Arun1 (AUTHOR), Khan, Mohd. Imran K.1 (AUTHOR), Govindaraju, Gayathri2 (AUTHOR), Verma, Mamta1 (AUTHOR), Awasthi, Sharad1 (AUTHOR), Chavali, Pavithra L.3 (AUTHOR), Chavali, Sreenivas4 (AUTHOR), Rajavelu, Arumugam1,2 (AUTHOR) arajavelu@rgcb.res.in, Dhayalan, Arunkumar1 (AUTHOR) arun.dbt@pondiuni.edu.in |
| المصدر: |
BBA - Molecular Cell Research. Feb2020, Vol. 1867 Issue 2, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*PROTEIN synthesis, *SITE-specific mutagenesis, *DNA methyltransferases, *PROTEIN-protein interactions, *RIBOSOMAL proteins, *METHYLTRANSFERASES, *METHYLATION, *CATALYSIS |
| مستخلص: |
Methylation of proteins is emerging to be an important regulator of protein function. SET7/9, a protein lysine methyltransferase, catalyses methylation of several proteins involved in diverse biological processes. SET7/9-mediated methylation often regulates the stability, sub-cellular localization and protein-protein interactions of its substrate proteins. Here, we aimed to identify novel biological processes regulated by SET7/9 by identifying new interaction partners. For this we used yeast two-hybrid screening and identified the large subunit ribosomal protein, eL42 as a potential interactor of SET7/9. We confirmed the SET7/9-eL42 interaction by co-immunoprecipitation and GST pulldown studies. The N-terminal MORN domain of SET7/9 is essential for its interaction with eL42. Importantly, we identified that SET7/9 methylates eL42 at three different lysines - Lys53, Lys80 and Lys100 through site-directed mutagenesis. By puromycin incorporation assay, we find that SET7/9-mediated methylation of eL42 affects global translation. This study identifies a new role of the functionally versatile SET7/9 lysine methyltransferase in the regulation of global protein synthesis. Unlabelled Image • Little is known about the regulation and functional significance of ribosomal proteins methylation. • The protein lysine methyltransferase, SET7/9 interacts with the large ribosomal protein eL42. • SET7/9 methylates eL42 at three different lysine residues both in vitro and in vivo. • SET7/9 mediated methylation of eL42 affects the global translation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder