Structural Analysis of the Genes for Human Arylamine N-Acetyltransferases and Characterisation of Alternative Transcripts
| العنوان: | Structural Analysis of the Genes for Human Arylamine N-Acetyltransferases and Characterisation of Alternative Transcripts |
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| المؤلفون: | Sotiria Boukouvala, Edith Sim |
| المصدر: | Basic Toxicology. 96:343-351 |
| بيانات النشر: | Wiley, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Gene isoform, Transcription, Genetic, Arylamine N-Acetyltransferase, Molecular Sequence Data, Biology, Polyadenylation, Toxicology, Exon, Complementary DNA, Humans, Coding region, Gene, Expressed Sequence Tags, Pharmacology, Genetics, Genomic Library, Expressed sequence tag, Reverse Transcriptase Polymerase Chain Reaction, Alternative splicing, Intron, Chromosome Mapping, Exons, General Medicine, Molecular biology, Introns, Isoenzymes, Alternative Splicing, Genes, Sequence Alignment, Chromosomes, Human, Pair 8 |
| الوصف: | Arylamine N-acetyltransferases are polymorphic drug-metabolising enzymes. The human isoforms, NAT1 and NAT2, are encoded by two genes with intronless coding regions. Human NAT1 protein is found in many tissues, unlike NAT2 which is present predominantly in the intestine and liver. We describe the exon-intron structure of the human NAT genes by analysing data from genomic databases. Comparison of expressed sequence tags, matching NAT gene sequences, with the sequence of human chromosome 8 implied the presence of 8 non-coding exons located 51.5, 51.4, 12.3, 11.9, 10.8, 9.6, 5.2 and 2.6 kb upstream of the single coding exon of the NAT1 gene. A number of expressed sequence tags also indicated transcription initiation from the upstream region adjacent to the NAT1 coding exon, consistent with earlier studies. The NAT2 gene consists of one previously described non-coding and one coding exon, located 8.6 kb apart. These findings were also confirmed by RT-PCR, using cDNA from heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Alternatively spliced NAT1 transcripts were found in all tissues. Transcription of the NAT2 gene was also detected in these tissues and was demonstrated to start either from the non-coding exon or from immediately upstream of the coding exon. Comparison of the RT-PCR products provided an initial estimate of the relative amounts of the different NAT transcripts expressed in each tissue. Finally, both expressed sequence tag analysis and RT-PCR demonstrated the presence of two differentially utilised polyadenylation signals for NAT1 and NAT2, located about 0.2 and 0.3 kb downstream of the coding region of each gene. |
| تدمد: | 1742-7843 1742-7835 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2556fe0284a9ec17e11f5ad2f56301d8Test https://doi.org/10.1111/j.1742-7843.2005.pto_02.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....2556fe0284a9ec17e11f5ad2f56301d8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17427843 17427835 |
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