دورية
Elevated ATG5 expression in autoimmune demyelination and multiple sclerosis
| العنوان: | Elevated ATG5 expression in autoimmune demyelination and multiple sclerosis |
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| المؤلفون: | Alirezaei, Mehrdad, Fox, Howard S., Flynn, Claudia T., Moore, Craig S., Hebb, Andrea L.O., Frausto, Ricardo F., Bhan, Virender, Kiosses, William B., Whitton, J. Lindsay, Robertson, George S., Crocker, Stephen J. |
| المصدر: | Autophagy; February 2009, Vol. 5 Issue: 2 p152-158, 7p |
| مستخلص: | Multiple sclerosis (MS) is an inflammatory central nervous system (CNS) disorder characterized by T cell mediated demyelination. In MS, prolonged T cell survival and increased T cell proliferation have been linked to disease relapse and progression. Recently, the autophagy related gene 5 (Atg5) has been shown to modulate T cell survival. In this study, we examined the expression of Atg5 using both a mouse model of autoimmune demyelination as well as blood and brain tissues from MS cases. Quantitative real-time PCR analysis of RNA isolated from blood samples of experimental autoimmune encephalomyelitis (EAE) mice revealed a strong correlation between Atg5 expression and clinical disability. Analysis of protein extracted from these cells confirmed both upregulation and post-translational modification of Atg5 the latter of which was positively correlated with EAE severity. Analysis of RNA extracted from T cells isolated by negative selection, indicated that Atg5 expression was significantly elevated in individuals with active relapsing-remitting MS compared to non-diseased controls. Brain tissue sections from relapsing-remitting MS cases examined by immunofluorescent histochemistry suggested that encephalitogenic T cells are a source of Atg5 expression in MS brain samples. Together these data suggest that increased T cell expression of Atg5 may contribute to inflammatory demyelination in MS. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 15548627 15548635 |
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| DOI: | 10.4161/auto.5.2.7348 |