The Presence of an Anti-Erythrocyte Autoantibody in C3HeB/FeJ mice after Lymphocytic Choriomeningitis Virus Infection
| العنوان: | The Presence of an Anti-Erythrocyte Autoantibody in C3HeB/FeJ mice after Lymphocytic Choriomeningitis Virus Infection |
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| المؤلفون: | Anthony T. Vella, Charles J. Pfau |
| المصدر: | Autoimmunity. 9:319-329 |
| بيانات النشر: | Informa UK Limited, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Male, Hemolytic anemia, Erythrocytes, Anemia, Immunology, Cross Reactions, Lymphocytic Choriomeningitis, medicine.disease_cause, Lymphocytic choriomeningitis, Mice, Antigen, Antibody Specificity, hemic and lymphatic diseases, medicine, Animals, Immunology and Allergy, Autoantibodies, Mice, Inbred C3H, Arenavirus, biology, Autoantibody, biology.organism_classification, medicine.disease, Virology, Immunoglobulin Isotypes, Molecular mimicry, biology.protein, Female, Antibody |
| الوصف: | Lymphocytic Choriomeningitis (LCM) virus, substrain Docile, causes a chronic infection in adult C3HeB/FeJ mice. The virus also induces a severe anemia which, unlike the viremia, eventually resolves. Initially, there is frank bone marrow deficit, but the anemia persists well beyond a strong erythroid compensatory response. An immune-mediated basis for the hemolytic anemia was suggested by its abrogation in cyclophosphamide-treated mice, as well as an abnormal number of spherocytes in the circulation. We now show by ELISA assay, using either anti-mouse Ig or RBC membrane ghosts as catching antigen, that unusually high quantities of antibodies can be eluted from the RBCs of virus-infected mice. Furthermore, the high transient antibody concentration correlates with the severity of the anemia. With no evidence for complement playing a role in the anemia, these data indicate that erythrophagocytosis (via macrophage FcRs) may be the mechanism for RBC elimination. The possibility of molecular mimicry (antibody cross-reactivity between LCM and RBC membrane epitopes) was considered but appeared unlikely since the RBC antibody eluates gave no signal in an LCM-specific ELISA (which showed an ever increasing serum titer of virus-specific antibody). Isotype determination of the RBC eluates revealed the following: IgG2a much greater than IgG1 greater than IgG2b greater than IgG3 greater than IgM. The precise role, if any, of LCM-virus induced polyclonal activation (most strikingly in the IgG2a subclass) has yet to be determined. |
| تدمد: | 1607-842X 0891-6934 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f04eb64d86e3fd336bf6e7ab930400c0Test https://doi.org/10.3109/08916939108997134Test |
| رقم الانضمام: | edsair.doi.dedup.....f04eb64d86e3fd336bf6e7ab930400c0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1607842X 08916934 |
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