Free DNA precipitates calcium phosphate apatite crystals in the arterial wall in vivo
| العنوان: | Free DNA precipitates calcium phosphate apatite crystals in the arterial wall in vivo |
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| المؤلفون: | Jérémy Sadoine, Michel Daudon, Jean-Baptiste Michel, Catherine Chaussain, Marie Bensussan, Raphael Coscas, Marie-Paule Jacob, Liliane Louedec, Ziad A. Massy, Dominique Bazin |
| المساهمون: | Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Infection et inflammation (2I), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de chirurgie vasculaire [AP-HP Ambroise-Paré, Boulogne-Billancourt], Hôpital Ambroise Paré [AP-HP], Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Picardie Jules Verne (UPJV), Service Néphrologie/Dialyse [AP-HP Ambroise-Paré], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Ambroise Paré [AP-HP], Pathologies, Imagerie et Biothérapies oro-faciales (EA 2496), Université Paris Descartes - Paris 5 (UPD5), Service d'Explorations fonctionnelles multidisciplinaires [CHU Tenon], CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Des Maladies Rénales Rares aux Maladies Fréquentes, Remodelage et Réparation, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Spectroscopie, Modélisation, Interfaces pour L'Environnement et la Santé (SMiLES), Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Solides (LPS), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), HAL-UPMC, Gestionnaire, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Sud - Paris 11 (UP11)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU) |
| المصدر: | Atherosclerosis Atherosclerosis, 2017, 259, pp.60-67. ⟨10.1016/j.atherosclerosis.2017.03.005⟩ Atherosclerosis, Elsevier, 2017, 259, pp.60-67. ⟨10.1016/j.atherosclerosis.2017.03.005⟩ |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Calcium Phosphates, Pathology, medicine.medical_specialty, chemistry.chemical_element, 030204 cardiovascular system & hematology, Calcium, Calcification, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Apatites, Spectroscopy, Fourier Transform Infrared, medicine, Animals, Humans, Human atherosclerosis, Vascular Calcification, TUNEL assay, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Elastase, Colocalization, Arteries, DNA, X-Ray Microtomography, medicine.disease, Aneurysm, Free DNA, Calcium score, 3. Good health, Rats, Arterial calcification, Disease Models, Animal, 030104 developmental biology, Smooth muscle cell, chemistry, Microscopy, Electron, Scanning, Cardiology and Cardiovascular Medicine, Crystallization, Cell-Free Nucleic Acids, Immunostaining, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience; Background and aimsThe arterial wall calcium score and circulating free DNA levels are now used in clinical practice as biomarkers of cardiovascular risk. Calcium phosphate apatite retention in the arterial wall necessitates precipitation on an anionic platform. Here, we explore the role of tissue-free DNA as such a platform.MethodsThe first step consisted of histological observation of samples from human and rat calcified arteries. Various stains were used to evaluate colocalization of free DNA with calcified tissue (alizarin red, fluorescent Hoechst, DNA immunostaining and TUNEL assay). Sections were treated by EDTA to reveal calcification background. Secondly, a rat model of vascular calcifications induced by intra-aortic infusions of free DNA and elastase + free DNA was developed. Rat aortas underwent a micro-CT for calcium score calculation at 3 weeks. Rat and human calcifications were qualitatively characterized using μFourier Transform Infrared Spectroscopy (μFTIR) and Field Emission-Scanning Electron Microscopy (FE-SEM).ResultsOur histological study shows colocalization of calcified arterial plaques with free DNA. In the intra-aortic infusion model, free DNA was able to penetrate into the arterial wall and induce calcifications whereas no microscopic calcification was seen in control aortas. The calcification score in the elastase + free DNA group was significantly higher than in the control groups. Qualitative evaluation with μFTIR and FE-SEM demonstrated typical calcium phosphate retention in human and rat arterial specimens.ConclusionsThis translational study demonstrates that free DNA could be involved in arterial calcification formation by precipitating calcium phosphate apatite crystals in the vessel wall. |
| وصف الملف: | application/pdf |
| تدمد: | 1879-1484 0021-9150 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::795b0d7b9a8b3c256a4cf33118219dc1Test https://pubmed.ncbi.nlm.nih.gov/28292668Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....795b0d7b9a8b3c256a4cf33118219dc1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18791484 00219150 |
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