دورية أكاديمية
Effects of nandrolone decanoate on expression of steroidogenic enzymes in the rat testis
| العنوان: | Effects of nandrolone decanoate on expression of steroidogenic enzymes in the rat testis |
|---|---|
| المؤلفون: | TaeSun Min, Ki-Ho Lee |
| المصدر: | Asian-Australasian Journal of Animal Sciences, Vol 31, Iss 5, Pp 658-671 (2018) |
| بيانات النشر: | Asian-Australasian Association of Animal Production Societies, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Animal culture LCC:Animal biochemistry |
| مصطلحات موضوعية: | Testis, Male Fertility, Steroidogenic Enzymes, Leydig Cell, Gene Expression, Nandrolone Decanoate, Animal culture, SF1-1100, Animal biochemistry, QP501-801 |
| الوصف: | Objective Nandrolone decanoate (ND) is an anabolic-androgenic steroid frequently used for clinical treatment. However, the inappropriate use of ND results in the reduction of serum testosterone level and sperm production. The suppressive effect of ND on testosterone production has not been investigated in detail. The present study was designed to examine the effect of ND on the expression of steroidogenic enzymes in the rat testis. Methods Male Sprague Dawley rats at 50 days of age were subcutaneously administrated with either 2 or 10 mg of ND/kg body weight/week for 2 or 12 weeks. The changes of transcript and protein levels of steroidogenic enzymes in the testis were determined by real-time polymerase chain reaction and western blotting analyses, respectively. Moreover, immunohistochemical analysis was employed to determine the changes of immunostaining intensity of these enzymes. The steroidogenic enzymes investigated were steroidogenic acute regulatory protein, cytochrome P450 side chain cleavage enzyme, 17α-hydroxylase, 3β-hydroxysteroid dehydrogenase, and cytochrome P450 aromatase. Results The treatment of ND resulted in depletion of Leydig cells and sloughing of germ cells in the testis. The ND treatment caused significant expressional decreases of steroidogenic enzymes at transcript and protein levels, and the destructive effects of ND on the testis were more apparent with a higher dose and a longer period of the treatment. Evident reduction of immunostaining intensity present in Leydig cells was clearly detected by the ND treatment. Conclusion The exposure to ND in young male results not only in histological changes of the testis but also in aberrant gene expression of testicular steroidogenic enzymes, consequently leading into the reduction of testosterone production in the testis and thus likely disruption of spermatogenesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1011-2367 1976-5517 |
| العلاقة: | http://www.ajas.info/upload/pdf/ajas-31-5-658.pdfTest; https://doaj.org/toc/1011-2367Test; https://doaj.org/toc/1976-5517Test |
| DOI: | 10.5713/ajas.17.0899 |
| الوصول الحر: | https://doaj.org/article/9b3eaec4025f4e0991f03d5f4d569c6dTest |
| رقم الانضمام: | edsdoj.9b3eaec4025f4e0991f03d5f4d569c6d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10112367 19765517 |
|---|---|
| DOI: | 10.5713/ajas.17.0899 |