Influence of a modified preservation solution in kidney transplantation: A comparative experimental study in a porcine model
| العنوان: | Influence of a modified preservation solution in kidney transplantation: A comparative experimental study in a porcine model |
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| المؤلفون: | Morva Tahmasbi Rad, Thomas Longerich, Marta M. Gebhard, Mohammad Golriz, Gani Kuttymuratov, Hamidreza Fonouni, Majid Esmaeilzadeh, Parvin Jarahian, Sepehr Abbasi, Alireza Faridar, Arianeb Mehrabi |
| المصدر: | Scipedia Open Access Scipedia SL Asian Journal of Surgery, Vol 40, Iss 2, Pp 106-115 (2017) |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Graft Rejection, Male, Adenosine, Swine, Hemodynamics, 030204 cardiovascular system & hematology, Potassium Chloride, chemistry.chemical_compound, Random Allocation, 0302 clinical medicine, Medicine, Insulin, Mannitol, Control parameters, Blood urea nitrogen, Kidney transplantation, ischemia reperfusion injury, Biopsy, Needle, Graft Survival, Pig model, Organ Preservation, preservation solution, Glutathione, Immunohistochemistry, Survival Rate, Treatment Outcome, 030220 oncology & carcinogenesis, Female, medicine.medical_specialty, Allopurinol, Organ Preservation Solutions, lcsh:Surgery, Urology, HTK solution, Sensitivity and Specificity, 03 medical and health sciences, Raffinose, Animals, Viaspan, Creatinine, business.industry, lcsh:RD1-811, medicine.disease, Kidney Transplantation, Surgery, Disease Models, Animal, Glucose, chemistry, business, Procaine |
| الوصف: | Summary Background/Objective Currently, due to lack of optimal donors, more marginal organs are transplanted. Therefore, there is a high interest to ameliorate preischemic organ preservation, especially for critical donor organs. In this regard, a new histidine-tryptophane ketoglutarate (HTK-N) solution has been designed and its protective efficacy was compared with the standard preservation solutions—University of Wisconsin solution and standard HTK or Custodiol (Bretschneider's solution). Methods Seventy-two landrace pigs were included into the study, as donors and recipients. The donor kidneys were perfused during explantation with cold University of Wisconsin solution ( n = 12), standard HTK ( n = 12), or HTK-N solutions ( n = 12), kept in the respective preservation solution at 4°C for 30 hours, implanted in the recipient pigs, and reperfused. The pigs survived in daily control for 7 days. The serum creatinine and blood urea nitrogen were assessed in pre- and postreperfusion phase on the 3 rd day and 7 th day posttransplantation. Additionally, tissue samples were taken to analyze the histopathological degree of tubular injury and regeneration before and after reperfusion. Results The three preservation groups were comparable in age, body weight, and hemodynamic parameters. According to statistical proof, they differed in none of the control parameters. Conclusion Although the new preservation HTK solution is in several points a well-thought-out modification of the standard HTK solution, its preservation efficacy, at least for kidney preservation in a pig model for 30 hours, seems to be comparable to the current used solutions. A real advantage, however, could be confirmed in clinical settings, where marginal organs may influence the clinical outcome. |
| تدمد: | 0219-3108 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24a778b4924590b78290453d20e35fadTest https://pubmed.ncbi.nlm.nih.gov/26337376Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....24a778b4924590b78290453d20e35fad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02193108 |
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