دورية أكاديمية
Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface
| العنوان: | Hypervirulent Klebsiella pneumoniae serotype K1 clinical isolates form robust biofilms at the air-liquid interface |
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| المؤلفون: | Cubero, Meritxell, Martí Martí, Sara, Domínguez Luzón, Ma. Ángeles (María Ángeles), González Díaz, Aida, Berbel, Dàmaris, Ardanuy Tisaire, María Carmen |
| المصدر: | Articles publicats en revistes (Medicina) |
| بيانات النشر: | Public Library of Science (PLoS) |
| سنة النشر: | 2019 |
| المجموعة: | Dipòsit Digital de la Universitat de Barcelona |
| مصطلحات موضوعية: | Klebsiella pneumoniae, Estudis de viabilitat, Feasibility studies |
| الوصف: | The prevalence of a new hypervirulent and hypermucoviscous K. pneumoniae phenotype (Hmv) is increasing worldwide, mainly linked to serotypes K1 and K2. Since capsular thickness can directly affect the capability to form biofilms, we aimed to evaluate the association between the Hmv phenotype with adhesion and biofilm formation in a collection of clinical K. pneumoniae isolates. We selected 38 Hmv clinical isolates [15 serotype K1; 9 serotype K2; 3 non-K1/K2 (rmpA+); 11 non-K1/K2 (rmpA-)] and 7 non-Hmv clinical isolates. The Hmv phenotype was assessed through the mucoviscosity test. Serum resistance was determined by bacterial viability tests in pooled human serum. Adhesion was evaluated with the Biofilm Ring Test®, and biofilm formation was identified by crystal violet staining (Solid-Liquid, SLI-biofilm) or visual examination (Air-Liquid, ALI-biofilm). This study linked for the first time the formation of robust ALI-biofilm plugs by K. pneumoniae to the capsular serotype K1, a group of hypervirulent strains which are generally highly susceptible to the antimicrobial agents. Among all the studied isolates, the capsular serotype K1 presented lower initial adhesion despite having the adhesins mrkD and fimH but higher ALI-biofilm formation than isolates with other capsular serotypes (K2 or non-K1/K2). This structure might confer increased resistance to a group of hypervirulent K. pneumoniae serotype K1. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 11 p.; application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0222628Test; PLoS One, 2019, vol. 14, num. 9, p. e0222628; https://doi.org/10.1371/journal.pone.0222628Test; http://hdl.handle.net/2445/176734Test; 709400 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0222628Test http://hdl.handle.net/2445/176734Test |
| حقوق: | cc-by (c) Cubero, Meritxell et al., 2019 ; http://creativecommons.org/licenses/by/3.0/esTest ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.4017D654 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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