Detection of herpes simplex-1 and -2 and varicella zoster virus by quantitative real-time polymerase chain reaction in corneas from patients with bacterial keratitis
| العنوان: | Detection of herpes simplex-1 and -2 and varicella zoster virus by quantitative real-time polymerase chain reaction in corneas from patients with bacterial keratitis |
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| المؤلفون: | Maria Cecilia Yu, Heloisa Nascimento, Ana Luisa Hofling-Lima, Paulo J.M. Bispo, Aripuana Watanabe, Celso Francisco Hernandes Granato, Andrea Pelegrini, Ana Carolina Vieira |
| المصدر: | Arquivos Brasileiros de Oftalmologia, Vol 80, Iss 2, Pp 84-87 Arquivos Brasileiros de Oftalmologia v.80 n.2 2017 Arquivos brasileiros de oftalmologia Conselho Brasileiro de Oftalmologia (CBO) instacron:CBO Arquivos Brasileiros de Oftalmologia, Volume: 80, Issue: 2, Pages: 84-87, Published: APR 2017 |
| بيانات النشر: | GN1 Genesis Network, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Herpesvirus 3, Human, Herpesvirus 2, Human, viruses, Reação em cadeia da polimerase, Herpesvirus 1, Human, medicine.disease_cause, Eye Infections, Bacterial, law.invention, Cornea, 0302 clinical medicine, lcsh:Ophthalmology, law, Medicine, Prospective Studies, Child, Ceratite herpética, Aged, 80 and over, General Medicine, Keratitis, Dendritic, Middle Aged, Polymerase chain reaction, Gram staining, Infecções por herpesviridae, Female, DNA Probes, Adult, Herpes simplex, Adolescent, Herpes simples, Dendritic Keratitis, Real-Time Polymerase Chain Reaction, Herpesviridae, Virus, Keratitis, Young Adult, 03 medical and health sciences, Humans, herpesviridae, Aged, business.industry, Varicella zoster virus, Eye infection, medicine.disease, Virology, Ophthalmology, Keratitis, herpetic, Herpes simplex virus, lcsh:RE1-994, 030221 ophthalmology & optometry, business, 030217 neurology & neurosurgery |
| الوصف: | Objective: Bacterial keratitis occurs worldwide, and despite recent developments, it remains a potentially blinding condition. This study assesses the presence of herpes simplex virus (HSV-1 and -2) and varicella zoster virus (VZV) by quantitative real-time polymerase chain reaction (qPCR) in corneal scrapings from patients with bacterial keratitis. Methods: A total of 65 patients with clinical diagnoses of infectious corneal ulcers prospectively underwent clinical eye examinations. Corneal scrapings were investigated by Gram staining, Giemsa staining, culture, and qPCR (the study group). Risk factors and epidemiological data were recorded. The control group comprising 25 eyes with typical herpes dendritic keratitis was also analyzed by qPCR. Results: From the study group (n=65), nine patients (13.8%) had negative smears, cultures, and qPCR findings. Fifty-six (86.2%) patients had positive cultures: 51 for bacteria, 4 for fungi, and 1 for amoebae. Of the patients who had positive bacterial cultures, qPCR identified 10 patients who were also positive for virus: one for VZV and nine for HSV-1. Of the 25 patients in the control group, 21 tested positive for HSV-1 by qPCR analysis. Conclusions: Herpes may be present in patients with bacterial corneal ulcers, and qPCR may be useful in its detection. RESUMO Objetivo: Ceratites bacterianas ocorrem mundialmente e apesar dos novos desenvolvimentos permanece como uma condição que pode levar à cegueira. Avaliar a presença de herpes simples (-1 e -2) e vírus varicella zoster (VZV) por reação em cadeia quantitativa de polimerase em tempo real (qPCR) em raspados corneanos de pacientes com ceratite bacteriana. Métodos: Sessenta e cinco pacientes com ceratite infecciosa foram submetidos a raspados corneanos estudados para gram, Giemsa, cultura e qPCR (grupo de estudo). Foram avaliados fatores de risco e epidemiológicos. O grupo controle foi composto por 25 casos de úlcera dendrítica típica por herpes analisados por qPCR. Resultados: Do grupo de estudo (n=65), nove pacientes (13,8%) apresentaram cultura, qPCR e raspado negativos. Cinquenta e seis (86,2%) pacientes apresentaram cultura positiva, 51 para bacteria, 4 para fungo e 1 para ameba. A qPCR identificou 10 pacientes do grupo de cultura positiva para bactéria que também foram positivos para vírus, um VZV e 9 para HSV-1. Dos 25 pacientes que compunham o grupo controle, 21 apresentaram qPCR positivo para HSV-1. Conclusão: Herpes pode estar presente em pacientes com úlceras de córnea bacterianas e a qPCR pode ser útil na sua detecção. |
| وصف الملف: | text/html |
| تدمد: | 0004-2749 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23d0eb319e1704d7f4ec0404f1d76dc7Test https://doi.org/10.5935/0004-2749.20170021Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....23d0eb319e1704d7f4ec0404f1d76dc7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00042749 |
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