التفاصيل البيبلوغرافية
| العنوان: |
A novel hepatitis C virus vaccine approach using recombinant Bacillus Calmette-Guerin expressing multi-epitope antigen. |
| المؤلفون: |
Wei, S.-H., Yin, W., An, Q.-X., Lei, Y.-F., Hu, X.-B., Yang, J., Lu, X., Zhang, H., Xu, Z.-K. |
| المصدر: |
Archives of Virology; Jun2008, Vol. 153 Issue 6, p1021-1029, 9p, 1 Black and White Photograph, 1 Diagram, 1 Chart, 5 Graphs |
| مصطلحات موضوعية: |
LIVER diseases, HEPATITIS C virus, LABORATORY mice, TUBERCULOSIS prevention, CELLULAR immunity |
| مستخلص: |
Hepatitis C virus (HCV) is a major cause of liver disease worldwide. HCV infection is associated with high morbidity and has become a major problem in public health. Until now, there has been no effective prophylactic or therapeutic vaccine. BCG, a live vaccine typically used for tuberculosis prevention, has been increasingly utilized as a vector for the expression of recombinant proteins that will induce specific humoral and cellular immune responses. In this study, recombinant BCG (rBCG) was engineered to express a HCV multi-epitope antigen CtEm, and HLA-A2.1 transgenic mice were immunized with rBCG-CtEm. High levels of specific anti-HCV antibodies targeted to mimotopes of HVR1 were detected in the serum. HCV-specific lymphocyte proliferation assay, cytokine determination and cytotoxicity assay indicated that HCV epotope-specific cellular immune responses were elicited in vitro. The rBCG-CtEm immunization conferred protection against infection with the recombinant vaccinia virus (rVV-HCV-CNS) in vivo. These results suggest that rBCG expressing multi-epitope antigen may serve as an effective vaccine against HCV infection. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
