Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2
| العنوان: | Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2 |
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| المؤلفون: | Duncan R. Smith, S Jindadamrongwech, Chutima Thepparit |
| المصدر: | Archives of Virology. 149:915-927 |
| بيانات النشر: | Springer Science and Business Media LLC, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Serotype, Receptor complex, Viral Plaque Assay, Dengue virus, medicine.disease_cause, Peptide Mapping, Mass Spectrometry, Virus, Cell Line, Dengue fever, Virology, medicine, Humans, Endoplasmic Reticulum Chaperone BiP, Heat-Shock Proteins, Immunoassay, biology, Binding protein, Liver cell, General Medicine, Dengue Virus, medicine.disease, biology.organism_classification, Molecular biology, Flavivirus, Liver, Receptors, Virus, Carrier Proteins, Molecular Chaperones |
| الوصف: | This study sought to identify receptor elements for dengue virus serotype 2 on human liver cells (HepG2) using the viral overlay protein binding assay (VOPBA) technique and Mass Spectrometry fingerprinting. A single major and several minor virus binding bands were observed, and mass spectrometry identified a candidate binding protein for the major binding band as GRP 78 (BiP). GRP78 expression on the cell surface was confirmed, and antibodies directed against both the N and C-terminus of GRP 78 (BiP) altered both the binding of the virus to the cell surface as well as the infectivity profile of HepG2 cells in response to dengue serotype 2 infection. GRP 78 (BiP), which has previously been identified as a co-receptor protein for coxsackievirus A9, is the first non-Fc receptor protein identified for the dengue virus, although GRP78 probably functions as part of a receptor complex. |
| تدمد: | 1432-8798 0304-8608 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9cb2b7a508376762341b2b152b9fe4bTest https://doi.org/10.1007/s00705-003-0263-xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....d9cb2b7a508376762341b2b152b9fe4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14328798 03048608 |
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