Liver-type of tissue non-specific alkaline phosphatase is induced during mouse bone and tooth cell differentiation
| العنوان: | Liver-type of tissue non-specific alkaline phosphatase is induced during mouse bone and tooth cell differentiation |
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| المؤلفون: | Shinichiro Oida, Tatsuo Terashima, Tomomi T. Baba |
| المصدر: | Archives of oral biology. 98 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Bone Morphogenetic Protein 2, Gene Expression, Bone morphogenetic protein, Bone and Bones, Cell Line, Myoblasts, 03 medical and health sciences, Mice, 0302 clinical medicine, Organ Culture Techniques, Osteogenesis, Gene expression, medicine, Animals, RNA, Messenger, General Dentistry, Gene, Messenger RNA, Osteoblasts, Chemistry, Tooth differentiation, Tooth Germ, Osteoblast, Bone-type TNSALP, Cell Differentiation, 030206 dentistry, Cell Biology, General Medicine, Alkaline Phosphatase, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Otorhinolaryngology, Liver, Cell culture, Liver-type TNSALP, Tissue non-specific alkaline phosphatase, Alkaline phosphatase, Female, C2C12 |
| الوصف: | Background and objective: Tissue non-specific alkaline phosphatase (TNSALP) contains two types?bone- and liver-type?which are produced from the same gene due to differences in splicing. These two differ in their promoter, but the amino acid sequences of the mature proteins are identical. In this study, we examined the relationship between the two types of TNSALP expression and osteoblast differentiation. Design: Gene expression of the two types of TNSALP was observed by reverse transcription-polymerase chain reaction. MC3T3-NM4 was sub-cloned from an established mouse osteoblastic cell line in which osteoblast characters do not appear without dexamethasone. The C2C12 mouse myoblastic cell line, which can be induced to osteoblasts with bone morphogenic protein 2, and organ-cultured tooth germs were also used in this work. Results: The gene expression of liver-type TNSALP was observed in only MC3T3-NM4 activated by dexamethasone. For C2C12, the gene expression of bone-type TNSALP was observed even in non-induced conditions where myotubes were formed, whereas the liver-type TNSALP mRNA was only expressed when C2C12 differentiated into osteoblasts by bone morphogenic protein 2. Furthermore, in the organ-cultured tooth germs, the liver-type TNSALP mRNA was expressed according to differentiation of tooth germs. Conclusion: These results suggest that the liver-type TNSALP mRNA is induced according to differentiation of bone and tooth. Archives of Oral Biology, 98, pp.32-37; 2019 |
| وصف الملف: | application/pdf |
| تدمد: | 1879-1506 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30160b0640eb1f628450ef6778e26d0aTest https://pubmed.ncbi.nlm.nih.gov/30439569Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....30160b0640eb1f628450ef6778e26d0a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18791506 |
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