Effects of zoledronic acid on odontoblast-like cells
| العنوان: | Effects of zoledronic acid on odontoblast-like cells |
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| المؤلفون: | Josimeri Hebling, Fernanda Gonçalves Basso, Ana Paula Silveira Turrioni, C. A. de Souza Costa |
| المساهمون: | Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (Unesp) |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| بيانات النشر: | Elsevier BV, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cytotoxicity, Cell morphology, Zoledronic Acid, zoledronic acid, odontoblast, dose response, bisphosphonic acid derivative, Cells, Cultured, bone density conservation agent, Protein Synthesis Inhibitors, Extracellular Matrix Proteins, Bone Density Conservation Agents, Diphosphonates, Odontoblasts, Chemistry, drug effect, scleroprotein, sialoglycoprotein, Imidazoles, Bisphosphonates, gene expression regulation, General Medicine, phosphoprotein, Succinate Dehydrogenase, Dose–response relationship, Alkaline phosphatase, drug antagonism, Cell Survival, Sialoglycoproteins, Collagen Type I, Gene Expression Regulation, Enzymologic, Bovinae, stomatognathic system, dentin sialophosphoprotein, Humans, MTT assay, human, Viability assay, General Dentistry, collagen type 1, cell culture, Dose-Response Relationship, Drug, Dentistry(all), protein synthesis inhibitor, Cell Biology, succinate dehydrogenase, Alkaline Phosphatase, Phosphoproteins, Molecular biology, Odontoblast, Otorhinolaryngology, Cell culture, imidazole derivative, biosynthesis, Fetal bovine serum |
| الوصف: | Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:29:01Z No. of bitstreams: 0 Made available in DSpace on 2014-05-27T11:29:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-05-01 The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd. UNICAMP-Universidade de Campinas Piracicaba School of Dentistry, Piracicaba, SP, 13414-903 UNESP-University Estadual Paulista Araraquara School of Dentistry, Araraquara, SP, 14801-903 UNESP-University Estadual Paulista Araraquara School of Dentistry, Araraquara, SP, 14801-903 |
| تدمد: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2012.09.016 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21979f53f51d2e66e798d91a9cc73a1aTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....21979f53f51d2e66e798d91a9cc73a1a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00039969 |
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| DOI: | 10.1016/j.archoralbio.2012.09.016 |