Enhanced Biosynthesis of D-Arabitol by Metschnikowia reukaufii Through Optimizing Medium Composition and Fermentation Conditions
| العنوان: | Enhanced Biosynthesis of D-Arabitol by Metschnikowia reukaufii Through Optimizing Medium Composition and Fermentation Conditions |
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| المؤلفون: | Junhua Yun, Xiaolan Li, Hossain M. Zabed, Xianghui Qi, Yufei Zhang, Yingfeng An, Jiaqi Huang, Mei Zhao, Yuvaraj Ravikumar, Guoyan Zhang |
| المصدر: | Applied Biochemistry and Biotechnology. 194:3119-3135 |
| بيانات النشر: | Springer Science and Business Media LLC, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | D-arabitol, Bioengineering, General Medicine, Metschnikowia reukaufii, Applied Microbiology and Biotechnology, Biochemistry, chemistry.chemical_compound, Biosynthesis, chemistry, Composition (visual arts), Fermentation, Food science, Molecular Biology, Biotechnology |
| الوصف: | D-arabitol is an important functional sugar alcohol, which can be used in the preparation of foods, chemicals, and medicines. Despite biological production of D-arabitol from low-cost substrates has recently been the focus of research, low yield of this technology has limited its large-scale exploitation. Optimization of this bioprocess could be a promising option to improve the yield of D-arabitol. In this study, one-factor-at-a-time (OFAT) strategy and Box-Behnken design (BBD) were used to increase D-arabitol production by Metschnikowia reukaufii CICC 31858 through optimizing the fermentation conditions and medium composition. The OFAT optimization provided the optimal conditions for temperature, agitation speed, and fermentation time of 30℃, 220 rpm, and 144 h, respectively. Likewise, the optimum concentrations of peptone, ammonium sulfate, KH2PO4, MgSO4·7H2O, and fumaric acid in the fermentation medium were (g/L) 7.5, 1, 2 , 0.5 and 7.5, respectively. Under these optimum conditions, 80.43 g/L of D-arabitol was produced from 200 g/L of glucose, with a productivity of 0.56 g/L/h. The BBD optimization with three important components of fermentation medium (KH2PO4, MgSO4·7H2O and fumaric acid) showed that the predicted titer of D-arabitol varied from 47.21 g/L to 89.27 g/L, and the actual titer of D-arabitol ranged from 47.36 to 89.83 g/L. The optimum concentrations (g/L) of KH2PO4, MgSO4·7H2O, and fumaric acid in the fermentation medium were found to be 1.0, 0.5, and 4.7g/L, respectively. Under the optimum conditions, 92.45 g/L of D-arabitol was finally produced with the yield and productivity of 0.46 g/g and 0.64 g/L/h, respectively. |
| تدمد: | 1559-0291 0273-2289 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0dc8c6a50c88a04b3619b851e7c9417Test https://doi.org/10.1007/s12010-022-03910-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f0dc8c6a50c88a04b3619b851e7c9417 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15590291 02732289 |
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