Identification and characterization of the proBA operon of Streptococcus bovis
| العنوان: | Identification and characterization of the proBA operon of Streptococcus bovis |
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| المؤلفون: | A L Basso, L Ferrara, Margherita Sacco, Ezio Ricca, C Campanile, M De Felice, Giuseppe Forlani, Rosangela Marasco |
| المساهمون: | Campanile, C, Forlani, G, Basso, A, Marasco, Rosangela, Ricca, E, Sacco, Margherita, Ferrara, L. AND DE FELICE M., Basso, Al, Marasco, R, Ricca, Ezio, Sacco, M, Ferrara, L, DE FELICE, M., DE FELICE, Maurilio |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | American Society for Microbiology, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | DNA, Bacterial, Enzyme complex, Proline, Operon, DNA, Recombinant, medicine.disease_cause, Applied Microbiology and Biotechnology, Plasmid, Escherichia coli, medicine, Genomic library, Gene Library, Genetics, Ecology, biology, Phosphotransferases, Streptococcus bovis, biology.organism_classification, Complementation, genomic DNA, Biochemistry, Oxidoreductases, Research Article, Food Science, Biotechnology |
| الوصف: | A genomic DNA library of the rumen bacterium Streptococcus bovis was constructed in Escherichia coli, and recombinant plasmids able to complement proA and proB mutations of the host were found. Southern hybridization and restriction analysis showed that a 3.5-kb fragment of S. bovis DNA contained two genes, organized in an operon and coding for enzymes functionally similar to the glutamyl phosphate reductase-glutamyl kinase enzyme complex that in E. coli catalyzes the first step of proline biosynthesis. Complementation of the E. coli mutations was observed with the fragment inserted in both orientations, which suggested that the S. bovis proBA operon was transcribed from its own promoter. Genetic and biochemical data suggested that the proline biosynthetic pathway of S. bovis is similar to the one previously characterized for E. coli. |
| تدمد: | 1098-5336 0099-2240 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dca05bd5a5eaa30b4f91da8c7b2a06dTest https://doi.org/10.1128/aem.59.2.519-522.1993Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6dca05bd5a5eaa30b4f91da8c7b2a06d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985336 00992240 |
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